Liver homogenate-mediated mutagenesis in chinese hamster V79 cells by polycyclic aromatic hydrocarbons and aflatoxins
Several chemical carcinogens that require metabolic activation have been examined for their cytotoxic and mutagenic activity in Chinese hamster V79 cells. Mutagenic activity was measured as the induced frequency of 6-thioguanine-resistant colonies. Metabolic activation was provided by the 9000 g supernatant fraction of rat liver plus cofactors. The cytotoxicity and mutagenicity of aflatoxin B1 and B2, benzo(a)pyrene, 3-methylcholanthrene, 7,12-dimethylbenz(a)anthracene, dibenz(a,h)-anthracene, dibenz(a,c)anthracene, and benz(a)anthracene were examined as functions of concentration. Except for the two isomers of dibenzanthracene, the mutagenic activity in general paralleled the carcinogenic activity. An assay of this type may be useful as a prescreen for environmental chemicals that require metabolic activation.
Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test
In vitro metabolic activation of chemical mutagens. I. Development of an in vitro mutagenicity assay using liver microsomal enzymes for the activation of dimethylnitrosamine to a mutagen
The influence of serum components on the growth and mutation of Chinese hamster cells in medium containing 8-azaguanine
Mutability of different genetic loci in mammalian cells by metabolically activated carcinogenic polycyclic hydrocarbons
Quantitative relationship between carcinogenicity and mutagenicity of polyaromatic hydrocarbons in Salmonella typhimurium mutants
Mutations to azaguanine resistance induced in cultured diploid human fibroblasts by the carcinogen, N-acetoxy-2-acetylaminofluorene
Reversion of the 8-azaguanine resistant phenotype of variant Chinese hamster cells treated with alkylating agents and 5-brono-2'-deoxyuridine
Mutagenicity of dimethylnitrosamine to mammalian cells as determined by the use of mouse liver microsomes
Determination of protein: a modification of the Lowry method that gives a linear photometric response
Carcinogens are mutagens: a simple test system combining liver homogenates for activation and bacteria for detection
Chemically induced mutation of L-5178Y mouse leukemia cells from asparagine-dependence to asparagine-independence
Carcinogens as frameshift mutagens: metabolites and derivatives of 2-acetylaminofluorene and other aromatic amine carcinogens
The effect of metabolic activation with rat liver preparations on the mutagenicity of several N-nitrosamines on a streptomycin-dependent strain of Escherichia coli
Somatic cell mutation. Detection and quantification of x-ray-induced mutation in cultured, diploid human fibroblasts
The induction of azaguanine-resistant mutants in cultured Chinese hamster cells by reactive derivatives of carcinogenic hydrocarbons
The influence of serum components on the growth and mutation of Chinese hamster cells in medium containing aminopterin
The in vivo and in vitro induction of aryl hydrocarbon hydroxylase in mammalian cells of different species, tissues, strains, and developmental and hormonal states
Metabolism and macromolecular binding of dibenz(a,c)anthracene and dibenz(a,h)anthracene by mouse embryo cells in culture
Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells
The effect of oral phenobarbitone on hepatic microsomal cytochrome P-450 and demethylation activity in rats fed normal and low protein diets
Use of an established human hepatoma cell line with endogenous bioactivation for gene mutation studies
Human bioassays to assess environmental genotoxicity: development of a DNA repair assay in HepG2 cells
Mutagenicity of dimethylnitrosamine and ethyl methanesulfonate as determined by the host-mediated CHO/HGPRT assay
Some factors determining the concentration of liver proteins for optimal mutagenicity of chemicals in the Salmonella/microsome assay
Facilitation by pyrimidine deoxyribonucleosides and hypoxanthine of mutagenic and cytotoxic effects of monofunctional alkylating agents in Chinese hamster cells
Further evidence that ouabain-resistant variants induced by chemical carcinogens in transformable C3H/10T1/2 Cl 8 mouse fibroblasts are mutants
Hamster hepatocyte-mediated activation of procarcinogens to mutagens in the L5178Y/TK mutation assay
Quantitative forward-mutation specificity of mono-functional alkylating agents, ICR-191, and aflatoxin B1 in mouse lymphoma cells
Modifying effects of the enzyme inducers, phenobarbital and 3-methylcholanthrene, on dominant lethal events induced by 7, 12-dimethylbenz[a]anthracene in mice
Rat liver homogenate (S9)-mediated binding of benzo[alpha]pyrene to DNA in V79 cells, V79 cell nuclei and aqueous solution
Validation and comparative studies on 180 chemicals with S. typhimurium strains and V79 Chinese hamster cells in the presence of various metabolizing systems
Mutagenesis by chemical agents in V79 chinese hamster cells: a review and analysis of the literature. A report of the Gene-Tox Program
The activation of procarcinogens to mutagens by cultured rat hepatocytes in the L5178Y/TK mutation assay
Induction of 6-thioguanine-resistant mutations by rat-liver homogenate (S9)-activated promutagens in human embryonic skin fibroblasts
Comparative genotoxic effects of the cooked-food-related mutagens Trp-P-2 and IQ in bacteria and cultured mammalian cells
Toxicity and mutagenicity of 6 anti-cancer drugs in Chinese hamster V79 cells co-cultured with rat hepatocytes
Induction of cytotoxicity and mutagenesis is facilitated by fatty acid hydroperoxidase activity in Chinese hamster lung fibroblasts (V79 cells)
Comparison between sister-chromatid exchange and mutagenicity following exogenous metabolic activation of promutagens
Higher efficiency of hamster hepatocytes than rat hepatocytes for detecting dimethylnitrosamine and diethylnitrosamine in hepatocyte-mediated Chinese hamster V79 cell mutagenesis assay
Cytotoxic effects of aflatoxin B1 and its association with cellular components in chicken embryo primary cultured cells
Polycyclic aromatic hydrocarbon in vitro cytotoxicity to bluegill BF-2 cells: mediation by S-9 microsomal fraction and temperature
Human bronchus-mediated mutagenesis of mammalian cells by carcinogenic polynuclear aromatic hydrocarbons
Chemical carcinogens produce mutations to ouabain resistance in transformable C3H/10T1/2 Cl 8 mouse fibroblasts
Characterization of c-Ki-ras oncogene alleles by direct sequencing of enzymatically amplified DNA from carcinogen-induced tumors
Introduction of cytochrome P450IA2 metabolic capability into cell lines genetically matched for DNA repair proficiency/deficiency
Comparison between carcinogenicity and mutagenicity based on chemicals evaluated in the IARC monographs
Biphenyl and fluorinated derivatives: liver enzyme-mediated mutagenicity detected in Salmonella typhimurium and Chinese hamster V79 cells
Promutagen activation by rodent-liver postmitochondrial fractions in the L5178Y/TK cell mutation assay
Inhibition of benzopyrene-diol-epoxide (BPDE)-induced bax and caspase-9 by cadmium: role of mitogen activated protein kinase
V79-hCYP2E1-hSULT1A1, a cell line for the sensitive detection of genotoxic effects induced by carbohydrate pyrolysis products and other food-borne chemicals
DNA repair in Syrian hamster embryo cells treated with 7,12-dimethylbenz[a]anthracene and its weakly carcinogenic 5-fluoro analog
Quantitative analysis of cytotoxicity and mutagenicity of benzo[a]pyrene in mammalian cells (CHO/HGPRT system)
Differential effect of manool--a diterpene from Salvia officinalis, on genotoxicity induced by methyl methanesulfonate in V79 and HepG2 cells
A comparison of two cytotoxicity assays for the detection of metabolism-mediated toxicity in vitro: a study with cyclophosphamide
Detection of DNA single-strand breaks induced by procarcinogens in Chinese hamster ovary cells cocultured with rat hepatocytes
Mutagenic and toxic activity of environmental effluents from underground coal gasification experiments
Cytotoxicity and mutagenicity of dimethylnitrosamine in mammalian cells (CHO/HGPRT system); enhancement by calcium phosphate
The use of primary rat hepatocytes to achieve metabolic activation of promutagens in the Chinese hamster ovary/hypoxanthine-guanine phosphoribosyl transferase mutational assay
The use of adult rat liver cultures in the detection of the genotoxicity of various polycyclic aromatic hydrocarbons
Chemically-induced unscheduled DNA synthesis in primary rat hepatocyte cultures: a comparison with bacterial mutagenicity using 218 compounds
Direct or proximate contact between cells and metabolic activation systems is required for mutagenesis
Lack of in vivo and in vitro genotoxicity with the nonsteroid, antiinflammatory agent sodium meclofenamate
Cell specificity in metabolic activation of aflatoxin B1 and benzo(a)pyrene to mutagens for mammalian cells
Absence of in vitro genotoxicity of pyrvinium pamoate in sister-chromatid exchange, chromosome aberration, and HGPRT-locus mutation bioassays
A benzo[a]pyrene-induced cell cycle checkpoint resulting in p53-independent G1 arrest in 3T3 fibroblasts.
Stable expression of human cytochrome P450IA2 cDNA in a human lymphoblastoid cell line: role of the enzyme in the metabolic activation of aflatoxin B1
Cytotoxicity and action mechanisms of polycyclic aromatic hydrocarbons by a miniature electrochemical detection system.
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