Liver metastatic model for human gastric cancer established by orthotopic tumor cell implantation

World Journal of Surgery
K YamaguchiK Hirata

Abstract

We have established an orthotopic implantation model that is highly metastatic to the liver. A human gastric carcinoma cell line, AZ521, with low capacity for liver metastasis was implanted as a single-cell suspension in the stomach of nude mice. The tumor cells derived from a few liver metastatic foci were subsequently implanted orthotopically, and we established a cell line, AZH5G, by repeating the in vivo stepwise selection method. This metastasizing line (AZH5G) developed liver metastasis in seven of eight (87.5%) cases, whereas parental AZ521 developed in 3 of 20 (15.0%). The in vitro growth activities of AZH5G were lower than that of AZ521, although the in vivo tumorigenicity of AZH5G was clearly higher than that of AZ521. Motility assays demonstrated higher motility of AZH5G than of AZ521. Flow cytometric analysis demonstrated that the expression of alpha 6-integrin significantly decreased in AZH5G (4.9% +/- 4.1%) compared to in AZ521 (17.7% +/- 8.1%) (p < 0.05). The adhesive activity of AZH5G cells to laminin was lower than that of AZ521 cells. In contrast, the adhesive activity of AZH5G cells to fibronectin was clearly higher than that of AZ521 cells. These findings suggested that changes in the expression of integrins...Continue Reading

Citations

Mar 27, 2010·Surgery Today·Yoshihiro KakejiYoshihiko Maehara
Apr 16, 2013·Journal of the American College of Surgeons·Jasneet Singh BhullarVijay K Mittal
Oct 15, 2003·Microsurgery·Bertram IllertWolfgang Timmermann
Jun 1, 2007·Current Protocols in Pharmacology·Susan Jones-Bolin, Bruce Ruggeri
Sep 10, 2009·Cancer Research·Kiyoshi YoshimuraRichard D Schulick

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