PMID: 240858Oct 25, 1975

Liver microsomal expoxide hydrase. Solubilization, purification, and characterization.

The Journal of Biological Chemistry
A Y LuW Levin

Abstract

Epoxide hydrase was solubilized from liver microsomes of phenobarbital-treated rats by treatment with cholate and purified to apparent homogeneity by ammonium sulfate fractionation and column chromatography in the presence of the nonionic detergent Emulgen 911 on DEAE-cellulose and hydroxylapatite. The purified enzyme preparation had a single major band with a molecular weight of 53,000 to 54,000 on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Other studies indicated that in the absence of sodium dodecyl sulfate, purified epoxide hydrase exists as high molecular weight aggregates. The preparation was essentially free of heme and flavin, but still contained small amounts of lipids and Emulgen 911.

Related Concepts

Amino Acids
Metazoa
Metal Antagonists
9,10-Epoxypalmitic Acid Hydrase
Hydro-Lyases
Hydrogen-Ion Concentration
Kinetics
Males
Microsomes, Liver
Molecular Weight

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.