Liver protection by hypothermic perfusion at different temperatures during total vascular exclusion

Liver International : Official Journal of the International Association for the Study of the Liver
Sander DinantThomas M van Gulik

Abstract

In situ hypothermic perfusion (HP) can be applied to attenuate ischemia and reperfusion (I/R) injury during liver resection under total vascular exclusion (TVE). This study examines the protective effect of cooling by HP at 20 and 28 degrees C as compared with no HP during TVE in a porcine liver I/R model. Twenty-one pigs underwent 60 min TVE of the liver followed by 24 h reperfusion. HP was performed via the portal vein using ringerlactate solution of 4 degrees C. Pigs were assigned to three groups: TVE without HP (no-HP, n=9), TVE with HP at 28 degrees C (HP-28, n=6) and TVE with HP at 20 degrees C (HP-20, n=6). Perfusion volumes during TVE were 5.1+/-0.5 and 17.3+/-1.7 l in HP-28 and HP-20, respectively (P<0.05). Aspartate aminotransferase (AST) after 24 h reperfusion was 1172+/-440 U/l in no-HP as compared with 223+/-69 and 180+/-22 U/l in HP-28 and HP-20, respectively (P<0.05). No differences in liver function or histopathology were found between the HP-28 and HP-20 groups. HP at 20 degrees C is equally effective in preserving liver function and preventing hepatocellular injury under TVE as compared with HP at 28 degrees C. HP at 28 degrees C is advised, because of the lesser perfusion volume necessary for cooling of the l...Continue Reading

Associated Clinical Trials

Dec 26, 2011·Megan J. Reiniers

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Citations

Mar 12, 2013·Indian Journal of Gastroenterology : Official Journal of the Indian Society of Gastroenterology·Sanjay Govil
Nov 12, 2013·Journal of the American College of Surgeons·Megan J ReiniersThomas M van Gulik
Nov 5, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Rowan F van GolenMichal Heger
Mar 11, 2016·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·C CodonyJ Figueras
Jul 28, 2007·Digestive Surgery·Thomas M van GulikDirk J Gouma
Mar 24, 2012·Digestive Surgery·Lisette T HoekstraThomas M van Gulik
May 4, 2011·Acta Cirúrgica Brasileira·Tomaz de Jesus Maria Grezzana FilhoCarlos Otavio Corso
Aug 26, 2009·Acta Cirúrgica Brasileira·Tomaz de Jesus Maria Grezzana FilhoCarlos Otávio Corso
Nov 10, 2015·Molecular Medicine·Pim B OlthofRowan F van Golen

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