PMID: 9419372Feb 21, 1998Paper

Liver repopulation with xenogenic hepatocytes in B and T cell-deficient mice leads to chronic hepadnavirus infection and clonal growth of hepatocellular carcinoma

Proceedings of the National Academy of Sciences of the United States of America
J PetersenC E Rogler

Abstract

To investigate host and viral mechanisms determining hepadnaviral persistence and hepatocarcinogenesis, we developed a mouse model by transplanting woodchuck hepatocytes into the liver of mice that contain the urokinase-type plasminogen activator transgene (uPA) and lack mature B and T lymphocytes due to a recombination activation gene 2 (RAG-2) gene knockout. The woodchuck hepatocytes were transplanted via intrasplenic injection and were found to integrate into the recipient mouse liver cord structure. Normal adult woodchuck hepatocytes proliferated and reconstituted up to 90% of the uPA/RAG-2 mouse liver. uPA/RAG-2 mice containing woodchuck hepatocytes were infectable with woodchuck hepatitis virus (WHV) and showed WHV replication for at least 10 months with titers up to 1 x 10(11) virions per ml in the peripheral blood. WHV-infected hepatocytes from chronic carrier woodchucks also established a persistent infection in uPA/RAG-2 mice after an 8- to 12-week lag period of viremia. Although WHV envelope, core, and X proteins were produced in the uPA/RAG-2 mice, no inflammatory host immune response was observed in the liver of WHV-replicating mice. A first antiviral test demonstrated a greater than four orders of magnitude drop i...Continue Reading

References

Jan 1, 1992·Advances in Cancer Research·M A Buendia
Oct 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R P VemuruS Gupta
Jan 1, 1991·Current Topics in Microbiology and Immunology·C E Rogler
Jan 1, 1988·Microbiology and Immunology·Y YamashitaS Matsuda
Jan 1, 1987·Annual Review of Biochemistry·D Ganem, H E Varmus
May 1, 1983·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J ScottoC Brechot
Jan 1, 1995·Annual Review of Immunology·F V Chisari, C Ferrari
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·J A RhimR L Brinster
Mar 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S GuptaK K Bhargava
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·L G GuidottiF V Chisari
Jan 1, 1995·Intervirology·M Roggendorf, T K Tolle
Sep 1, 1996·The Medical Clinics of North America·M W Fried
Jan 1, 1995·Intervirology·G P LeonardiP Harris
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·B S Blumberg

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Citations

Aug 18, 2012·Seminars in Immunopathology·Maura DandriJörg Petersen
Sep 1, 2005·Surgery Today·Yujo KawashitaTakashi Kanematsu
Aug 7, 2003·Biochemical and Biophysical Research Communications·P MeulemanG Leroux-Roels
Apr 12, 2002·Journal of Hepatology·David A Shafritz, Mariana D Dabeva
Feb 28, 2002·Journal of Hepatology·Chandan Guha, Jayanta Roy Chowdhury
Apr 11, 2001·Toxicology·A Nieto-RodríguezL Rodríguez-Fragoso
Jun 30, 2004·Lab Animal·Chandan GuhaJayanta Roy-Chowdhury
Aug 10, 2007·Nature Biotechnology·David A Shafritz
Jun 28, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M DandriJ Petersen
Oct 5, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M DandriJ Petersen
Mar 1, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Volker BrassDarius Moradpour
Nov 16, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Valentina SalucciFabio Palombo
Jun 15, 2001·ILAR Journal·M A Feitelson, J D Larkin
Dec 16, 1998·Xenotransplantation·M Koulmanda, H Auchincloss
Jul 23, 2013·Virology Journal·Wolfram H Gerlich
Oct 25, 2013·PloS One·Abeba TesfayeStephen M Feinstone
Mar 7, 2008·The American Journal of Pathology·Fabio MarongiuEzio Laconi
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Karl-Dimiter BissigInder M Verma
Mar 19, 2014·Journal of Immunological Methods·Maura Dandri, Marc Lütgehetmann
Feb 20, 2016·Journal of Hepatology·Jérôme Gouttenoire, Darius Moradpour
Sep 1, 2009·Expert Opinion on Drug Metabolism & Toxicology·Katsutoshi Yoshizato, Chise Tateno
Jun 24, 2010·Pathologie-biologie·Y XieM Zhao
Dec 14, 2006·Clinics in Liver Disease·Chen Liu
Aug 4, 2004·Liver International : Official Journal of the International Association for the Study of the Liver·Jae-Jin ChoSanjeev Gupta
Mar 26, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Philip MeulemanGeert Leroux-Roels
Oct 27, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Masataka TsugeKatsutoshi Yoshizato
Apr 23, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Tomoko NagaseYoshiki Sasai
Aug 3, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Marc BrulportJan G Hengstler
Nov 28, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Bruno Christ, Sandra Pelz
Jun 26, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Marc LutgehetmannMaura Dandri
Feb 8, 2006·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·M DandriJ Petersen
Apr 15, 2003·Clinics in Liver Disease·Thomas Pietschmann, Ralf Bartenschlager
Apr 17, 2016·Journal of Hepatology·Lena Allweiss, Maura Dandri
Aug 11, 2000·Seminars in Cancer Biology·S Gupta
Sep 2, 2006·Drug Metabolism and Pharmacokinetics·Kimiko AokiKatsutoshi Yoshizato
May 7, 2008·Journal of Hepatology·Heidi BarthThomas F Baumert

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