Ljungan virus present in intrauterine fetal death diagnosed by both immunohistochemistry and PCR

Birth Defects Research. Part A, Clinical and Molecular Teratology
Annika SamsioeBo Niklasson

Abstract

Following up on prior evidence from animal and human studies of Ljungan virus (LV) in intrauterine fetal death (IUFD), we examine additional cases of IUFD using two standard assays of viral detection: immunohistochemistry (IHC) and real time RT-PCR. Frozen and formalin-fixed specimens from IUFD cases were tested for the presence of LV using real time RT-PCR and IHC, respectively. Formalin-fixed organs from terminated pregnancies diagnosed as trisomy 21 were used as controls in the IHC assay. Presence of LV was demonstrated in all five IUFD cases by IHC and further confirmed in three of these cases by real time RT-PCR. Only one of 18 trisomy 21 controls was LV positive by IHC. The presence of LV in IUFD patients has been confirmed by two different assays.

References

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Aug 9, 2006·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Annika SamsioeBo Niklasson
Mar 6, 2007·Birth Defects Research. Part A, Clinical and Molecular Teratology·Bo NiklassonWilliam Klitz
Apr 12, 2008·Journal of Virological Methods·Conny TolfA Michael Lindberg
Oct 14, 2008·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Annika SamsioeWilliam Klitz
Jan 31, 2009·Birth Defects Research. Part A, Clinical and Molecular Teratology·Bo NiklassonWilliam Klitz

Citations

May 15, 2012·Journal of Virological Methods·René KalliesMatthias Niedrig
Jul 16, 2013·Journal of Medical Virology·Anne J JääskeläinenOlli Vapalahti
Jan 31, 2009·Birth Defects Research. Part A, Clinical and Molecular Teratology·Bo NiklassonWilliam Klitz
Oct 5, 2010·Birth Defects Research. Part A, Clinical and Molecular Teratology·Henry F Krous, Neil E Langlois
Mar 20, 2015·Birth Defects Research. Part C, Embryo Today : Reviews·Lili ZhengHong Xin
Jun 27, 2020·JAD Reports·Bo NiklassonElisabet Englund
Jun 4, 2020·Vector Borne and Zoonotic Diseases·Cristina FevolaHeidi C Hauffe

Related Concepts

Brain
Fetal Mummification
Immunocytochemistry
Lung
Pregnancy Complications, Infectious
Spleen
Partial Trisomy
Nested Polymerase Chain Reaction
Parechovirus
Diagnosis

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