PMID: 9449242Aug 1, 1997Paper

LNCaP prostatic adenocarcinoma cells derived from low and high passage numbers display divergent responses not only to androgens but also to retinoids

The Journal of Steroid Biochemistry and Molecular Biology
M EsquenetG Verhoeven

Abstract

In the present paper, two strains of LNCaP cells derived from the same source (American Type Culture Collection), but studied either at a low passage number (LP) or at a high passage number (HP), were compared in their response to R1881 (a synthetic androgen), all-trans-retinoic acid (atRA), and 1alpha,25-dihydroxycholecalciferol (VD3). [3H]Thymidine incorporation and epidermal growth factor receptor (EGF-R) binding were measured as parameters related to the proliferative response of the cells. The secretion of prostate-specific antigen (PSA) and the mRNA expression of PSA, prostatic acid phosphatase (PAP), and diazepam-binding inhibitor (DBI) were used as parameters reflecting differentiated function. Marked differences were noted in the response of LP and HP cells to androgens. [3H]Thymidine incorporation displayed a bell-shaped dose-response curve in both strains. The amplitude of the response, however, was much higher in HP cells and growth inhibition at high levels of R1881 was only observed in LP cells. On the contrary, androgen induction of PSA secretion and PSA mRNA expression, as well as the expression of PAP was much more pronounced in LP cells, whereas DBI expression was not altered according to passage number. LP ce...Continue Reading

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Citations

Mar 24, 1998·Molecular and Cellular Endocrinology·M EsquenetG Verhoeven
Feb 28, 2002·The Journal of Steroid Biochemistry and Molecular Biology·Nigel CleggPeter S Nelson
Aug 12, 1998·The Journal of Steroid Biochemistry and Molecular Biology·J V Swinnen, G Verhoeven
Jun 8, 2011·Molecular Psychiatry·G KonopkaD H Geschwind
Apr 27, 2010·Pancreas·Emily L DeerSean J Mulvihill
Oct 6, 2012·Clinical Sarcoma Research·Alvin Jm NgCarl R Walkley
Sep 23, 2014·Artificial Intelligence in Medicine·Gopal Krishna DhondalayMichael Hoare
Jan 28, 2016·Analytical Chemistry·Stephanie M SchubertDavid R Walt
Jan 4, 2012·The Prostate·Lien SpansFrank Claessens
Aug 3, 2010·Biotechnology and Bioengineering·J P Acosta-MartinezM Hoare
Jun 23, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·David AzzopardiGary Phillips
Apr 23, 2015·Progress in Lipid Research·Ditte NeessNils J Færgeman
Jan 17, 2012·Molecular and Cellular Endocrinology·Sathish Kasina, Jill A Macoska
May 13, 2014·Advanced Drug Delivery Reviews·Stuart J EllemGail P Risbridger
Sep 3, 2013·Journal of Biophotonics·Patience MthunziJamie H Warner
Feb 9, 2018·Expert Opinion on Drug Discovery·Luca Mazzarella, Giuseppe Curigliano
Oct 8, 2017·In Vitro Cellular & Developmental Biology. Animal·Yvonne A Reid
Feb 18, 2016·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Jillian N EskraNur Özten
Apr 13, 2012·Molecular Cancer Therapeutics·Christine HelsenFrank Claessens
Jun 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Carmen JerónimoDavid Sidransky
Aug 20, 2019·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Hudson W CoatesAndrew J Brown
Feb 13, 2021·Journal of Environmental Science and Health. Part C, Toxicology and Carcinogenesis·Si ChenLei Guo
Apr 27, 2021·Experimental Biology and Medicine·Passley Hargrove-GrimesDanilo A Tagle
Mar 14, 2021·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Milankumar PrajapatiThomas B Bartnikas
Aug 28, 2021·Genes·Emel RothzergSulev Kõks

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