lncINS-IGF2 Promotes Cell Proliferation and Migration by Promoting G1/S Transition in Lung Cancer

Technology in Cancer Research & Treatment
Shenglan GaoGang Liu

Abstract

Long noncoding RNAs are capable of regulating gene expression at multiple levels. These RNA molecules are also involved in a variety of physiological and pathological processes. Emerging data demonstrate that a series of differentially expressed long noncoding RNAs are implicated in tumorigenesis. In the present study, we used microarray analysis to identify long noncoding RNAs that are dysregulated in non-small-cell lung cancer when compared to normal lung tissues. Accordingly, we performed quantitative real-time polymerase chain reaction to analyze the levels of long noncoding RNA and the cis target gene. We further found the oncogene property of long noncoding RNA that long noncoding RNA downexpression inhibits non-small-cell lung cancer cells proliferation and migration based on 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide and colony formation assays and wound healing as well as transwell assays. The influence of long noncoding RNA on cell cycle of non-small-cell lung cancer cells is also analyzed by flow cytometry. Among the dysregulated long noncoding RNAs, we identified INS-IGF2 readthrough, transcript variant 1, noncoding RNA (NR_003512.3) is upregulated in non-small-cell lung cancer tissues, the ci...Continue Reading

References

Sep 2, 1998·The International Journal of Biochemistry & Cell Biology·S D O'Dell, I N Day
Sep 21, 2000·Journal of the National Cancer Institute·H Yu, T Rohan
Feb 4, 2009·Nature Reviews. Genetics·Tim R MercerJohn S Mattick
Oct 16, 2009·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Pascal SèveCharles Dumontet
Apr 15, 2011·Molecular Cancer·Ewan A GibbWan L Lam
Nov 19, 2011·Cancer Discovery·John R Prensner, Arul M Chinnaiyan
Mar 1, 2012·Frontiers in Genetics·Courtney N NilandAhmad M Khalil
Jun 6, 2012·RNA Biology·Tony Gutschner, Sven Diederichs
Sep 22, 2012·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Peng Qi, Xiang Du
Nov 24, 2012·Nature Reviews. Cancer·Nicola J Curtin
Dec 22, 2012·Gerontology·Daniel BergmanWilhelm Engström
Jun 4, 2013·Journal of Hematology & Oncology·Hua ZhangYueqin Chen
Oct 2, 2013·Endocrine-related Cancer·Callum Livingstone
Jan 9, 2014·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
May 30, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Pritsana RaungrutParamee Thongsuksai
Nov 6, 2015·Nature Medicine·Maite Huarte
Dec 23, 2015·Journal of Genetics·Mina KazemzadehAyla Valinezhad Orang
Jan 9, 2016·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Oct 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Michael A RogersPeter Lichter
Sep 28, 2016·Journal of Experimental & Clinical Cancer Research : CR·Roberta ViscontiDomenico Grieco

❮ Previous
Next ❯

Citations

Oct 28, 2019·International Journal of Molecular Sciences·Vincenza BarresiDaniele Filippo Condorelli

❮ Previous
Next ❯

Methods Mentioned

BETA
chip
transfection
flow cytometry

Software Mentioned

Arraystar

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.