lncRA ANCR Inhibits Non-Small Cell Lung Cancer Cell Migration and Invasion by Inactivating TGF-β Pathway

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Shaobin WangYang Xia

Abstract

BACKGROUND lncRNA ANCR is proved to be a tumor suppressor gene only in colorectal cancer and breast cancer. Our study aimed to explore the possible involvement of ACNR in non-small cell lung cancer (NSCLC). MATERIAL AND METHODS In this study, we first detected the expression of ACNR in lung biopsies and plasma of both NSCLC patients and healthy controls. The diagnostic value of ANCR for NSCLC was analyzed by ROC curve analysis. Follow-up data of NSCLC patients was analyzed and the prognostic value of ANCR was analyzed by survival curve analysis. ANCR expression vector was transfected into cells of human NSCLC cell lines, and the effects on cell migration and invasion were explored by Transwell migration and invasion assays, respectively. TGF-β1 expression after ANCR overexpression was detected by Western blot analysis. RESULTS ANCR was significantly downregulated in NSCLC patients compared with healthy controls in lung biopsies and plasma. Downregulated expression of ANCR distinguishes NSCLC patients from healthy controls and low NSCLC expression level indicates shorter postoperative survival time of NSCLC patients. ANCR overexpression inhibited NSCLC cell migration and invasion and downregulated TGF-β1 expression, while TGF-β1...Continue Reading

Citations

Jun 8, 2019·Molecular Medicine Reports·Zhen DengJianming Tan
Feb 15, 2020·International Journal of Molecular Sciences·Xing-Ning LaiLi-Xia Xiong
Jun 15, 2019·International Journal of Molecular Sciences·Yang HaoPeter Ten Dijke
Feb 2, 2021·Molecular Cancer·Swati MohapatraGeorge A Calin

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Methods Mentioned

BETA
biospies
biopsy
PCR
transfection
electrophoresis
biopsies

Software Mentioned

GraphPad Prism
Image J

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