LncRNA AK139128 promotes cardiomyocyte autophagy and apoptosis in myocardial hypoxia-reoxygenation injury

Life Sciences
Zhidong Zhu, Chufeng Zhao

Abstract

Long noncoding RNA (lncRNAs) participate in various biological processes in a number of cardiovascular diseases. Recently, a novel lncRNA AK139128 was found to be differentially expressed in myocardial ischemia-reperfusion injury (MIRI) tissue compared to normal myocardial tissues. Its pathological role in MIRI however, remains unknown. The expression level of AK139128 was investigated by quantitative real-time polymerase chain reaction assays (qRT-PCR) in MIRI tissues and cardiomyocytes. The effects of AK139128 in cell autophagy and apoptosis were determined using TUNEL assay, immunofluorescence assay and western blotting. Bioinformatics analysis and luciferase reporter assay were performed to verify the target relationship between AK139128, miR-499 and FOXO4. Results show that AK139128 was significantly up-regulated in MIRI tissues and cardiomyocytes. Knockdown of AK139128 impressively alleviates cardiomyocyte autophagy and apoptosis. Bioinformatics analysis and luciferase reporter assay showed that miR-499 targeted both 3'UTR of AK139128 and FOXO4 mRNA. Further functional experiments showed that miR-499 inhibitor eliminates the effects of knockdown of AK139128 on apoptosis and autophagy. Taken together, results from this stu...Continue Reading

Citations

Jan 20, 2020·Journal of Cellular and Molecular Medicine·Lin CongMingyuan Huang
Apr 2, 2020·Dose-response : a Publication of International Hormesis Society·Jie YangChuan-Hua Yang
Feb 3, 2021·Autophagy·Soudeh Ghafouri-FardMohammad Taheri
Jun 22, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Taheri

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