LncRNA-ANRIL inhibits cell senescence of vascular smooth muscle cells by regulating miR-181a/Sirt1.

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
Pan TanHai-Qin Wang

Abstract

Cardiovascular disease is one of the major threats to human life and health, and vascular aging is an important cause of its occurrence. Antisense non-coding RNA in the INK4 locus (ANRIL) is a kind of long non-coding RNA (lncRNA) that plays important roles in cell senescence. However, the role and mechanism of ANRIL in senescence of vascular smooth muscle cells (VSMCs) are unclear. Cell viability and cell cycle were evaluated using an MTT assay and flow cytometry analysis, respectively. Senescence-associated (SA)-β-galactosidase (gal) staining was used to determine cell senescence. Dual luciferase reporter assays were conducted to confirm the binding of ANRIL and miR-181a, as well as miR-181a and Sirt1. The expression of ANRIL, miR-181a, and Sirt1 was determined using qRT-PCR and protein levels of SA-β-gal and p53-p21 pathway-related proteins were evaluated by Western blotting. ANRIL and Sirt1 were down-regulated, whereas miR-181a was up-regulated in aging VSMCs. In young and aging VSMCs, over-expression of ANRIL could down-regulate miR-181a and up-regulate Sirt1. MTT and SA-β-gal staining assays showed that over-expression of ANRIL and inhibition of miR-181a promoted cell viability and inhibited VSMC senescence. The dual-lucif...Continue Reading

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Citations

Apr 4, 2020·Journal of Biomedical Science·Chih-Fan YehKai-Chien Yang
May 29, 2020·Laboratory Investigation; a Journal of Technical Methods and Pathology·Huimin KongQiang Luo
Aug 7, 2019·IUBMB Life·Qidong CaoChunli Song
Jan 31, 2020·Oxidative Medicine and Cellular Longevity·Qidong CaoChunli Song
Oct 30, 2020·Frontiers in Pharmacology·Fang Wang, Hou-Zao Chen
Dec 18, 2020·Frontiers in Cardiovascular Medicine·Apurva ShrivastavaChristian Schulte
Nov 10, 2020·Journal of Cardiovascular Pharmacology·Xin ZhaoJie Xi
Feb 24, 2021·BioFactors·Sanam DolatiSarvin Sanaie
Aug 14, 2021·Frontiers in Cell and Developmental Biology·Tianbin ChenLi Zhang
Aug 10, 2021·Frontiers in Physiology·Qing TanJun Li
Feb 3, 2022·Journal of Cellular Biochemistry·Mohammad Heydarnezhad AslAhad Mokhtarzadeh

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