LncRNA BACE1-AS Promotes Autophagy-Mediated Neuronal Damage Through The miR-214-3p/ATG5 Signalling Axis In Alzheimer's Disease.

Neuroscience
Yuan ZhouQi Zhang

Abstract

Alzheimer's disease (AD) is the most common neurodegenerative disease and is characterized by progressive memory loss and cognitive dysfunction. Long non-coding RNAs (lncRNAs) have been shown to be among the most promising biomarkers and therapeutic targets of AD. Here, we aimed to investigate whether lncRNA BACE1-AS plays a role in the potential mechanisms of AD. The expression of BACE1-AS, miR-214-3p and ATG5 mRNA was detected using qRT-PCR. The expression of the LC3, P62, ATG5, Bcl-2, p-Tau and cleaved-caspase 3 proteins was examined using western blot analysis. Cell apoptosis, cytotoxicity and ROS levels were estimated using flow cytometry, an LDH kit and a DCFH-DA assay, respectively. The interaction between BACE1-AS or ATG5 and miR-214-3p was validated using a dual-luciferase reporter assay. HE staining and a TUNEL assay were employed to evaluate hippocampal neuronal injury. The BACE1-AS level was found to be upregulated in serum samples of AD patients, brain tissues of AD transgenic (Tg) mice and Aβ1-42-treated SH-SY5Y cells. Autophagy activity was increased in both Tg mice and Aβ1-42-treated cells. BACE1-AS knockdown alleviated Aβ1-42-induced cell injury. Rapamycin abolished the protective effects of sh BACE1-AS against...Continue Reading

References

Apr 10, 1992·Science·J A Hardy, G A Higgins
Mar 9, 2005·Journal of Neuropathology and Experimental Neurology·Ralph A NixonAna Maria Cuervo
Oct 6, 2005·The Journal of Cell Biology·W Haung YuRalph A Nixon
Oct 12, 2005·The Journal of Cell Biology·Noboru Mizushima
Jul 29, 2006·Autophagy·Ana Maria CuervoAlexei Terman
Jul 29, 2006·Autophagy·David C RubinszteinAviva Tolkovsky
Jan 24, 2013·Therapeutic Advances in Chronic Disease·Enrico Mossello, Elena Ballini
Mar 19, 2013·Cell·Pedro J Batista, Howard Y Chang
Jun 4, 2013·Antioxidants & Redox Signaling·Katherine R Parzych, Daniel J Klionsky
Jun 25, 2013·Cancer Letters·Xuefei ShiYong Song
Feb 26, 2015·Neurodegenerative Disease Management·Lynn M Bekris, James B Leverenz
Apr 15, 2015·Trends in Genetics : TIG·Georges St LaurentPhilipp Kapranov
Jun 3, 2016·Clinical Medicine : Journal of the Royal College of Physicians of London·Robert BriggsDesmond O'Neill
Jun 5, 2016·Cellular and Molecular Neurobiology·Qian LiMiao Sun
Jun 25, 2016·Current Alzheimer Research·Paola RivaMarco Venturin
May 26, 2017·Methods in Molecular Biology·Min XueBin Shan
Jul 14, 2017·Cancer Research·Arunoday BhanSubhrangsu S Mandal
Aug 30, 2017·Current Alzheimer Research·Priya GuptaChiranjib Chakraborty
Sep 4, 2017·Life Sciences·Palaniyandi RavananPriti Talwar
Sep 14, 2017·Alzheimer's Research & Therapy·Marta Crous-BouJosé Luis Molinuevo
Dec 14, 2017·Essays in Biochemistry·Jeanne Mialet-Perez, Cécile Vindis
Feb 10, 2018·The American Journal of Medicine·Seth A GaleKirk R Daffner
Aug 24, 2018·F1000Research·Jason Weller, Andrew Budson
Sep 25, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jindong WangBailing Wang
Jan 18, 2019·Bioscience, Biotechnology, and Biochemistry·Xinhua WangHengfang Liu
Jul 3, 2019·Journal of Molecular Neuroscience : MN·Seyedeh Nahid FotuhiMahnaz Talebi

❮ Previous
Next ❯

Citations

Aug 3, 2021·American Journal of Alzheimer's Disease and Other Dementias·Yuchen He, Yi Qiang
Aug 21, 2021·Journal of Biomedical Science·Chih-Wei TungShang-Hsun Yang
Aug 21, 2021·Journal of Molecular Neuroscience : MN·Soudeh Ghafouri-FardMohammad Samadian
Nov 7, 2021·Cellular and Molecular Life Sciences : CMLS·Rohan Gupta Pravir Kumar
Nov 9, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Sajad NajafiShahin Aghamiri

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Alzheimer's Disease: Animal Models

Alzheimer's disease is a neurodegenerative disease which can be studied using various experimental systems. This feed focuses on animal models used for Alzheimer's disease research.

Alzheimer's Disease: Tau & TDP-43

Alzheimer's disease is a neurodegenerative disease. This feed focuses on the underlying role of tau proteins and TAR DNA-binding protein 43, as well as other genetic factors, in Alzheimer's disease.

Autophagy & Disease

Autophagy is an important cellular process for normal physiology and both elevated and decreased levels of autophagy are associated with disease. Here is the latest research.