LncRNA CRNDE promotes hepatocellular carcinoma cell proliferation, invasion, and migration through regulating miR-203/ BCAT1 axis

Journal of Cellular Physiology
Degang JiHaiying Liang

Abstract

To investigate the impact of long noncodingRNA (lncRNA) colorectal neoplasia differentially expressed (CRNDE) on hepatocellular cancer (HCC) cell propagation, invasion, and migration by mediating miR-203/ BCAT1 axis. Microarray analysis was based on 25 pairs of HCC cancerous tissues and adjacent tissues. The expression levels of CRNDE, miR-203, and BCAT1 in HCC tissues were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). The liver cell line L-02 and HCC cell lines HepG2 and Huh-7 were utilized to assess the regulatory effects of CRNDE and miR-203 on HCC progression in vitro. Western blot was used to qualify BCAT1 protein expression level. Cell proliferation and apoptosis were evaluated using CCK-8 and flow cytometry analysis, whereas cell invasion and migration assay were performed by the Transwell assay. The relationship among CRNDE, miR-203, and BCAT1 was validated by dual luciferase assay. Tumor Xenograft study was established to verify the pathological effect of CRNDE on HCC development in vivo. The expression levels of the CRNDE and BCAT1 were upregulated in HCC tissues and cells, whereas miR-203 was downregulated in HCC. Knockdown of CRNDE or miR-203 overexpression would inhibit HCC cell propagation...Continue Reading

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Citations

Sep 3, 2020·Non-coding RNA·Shizuka Uchida, Sakari Kauppinen
May 22, 2020·Cancer Cell International·Ya LuJifeng Feng
Dec 30, 2020·International Journal of Molecular Sciences·Miguel Ángel Cáceres-DuránAmanda Ferreira Vidal
Jan 21, 2021·OncoTargets and Therapy·Lu RenJun Tian
May 11, 2021·Frontiers in Oncology·Soudeh Ghafouri-FardMohammad Taheri
May 29, 2021·Frontiers in Cell and Developmental Biology·Fan PanRui Wang
Jul 26, 2021·European Journal of Pharmacology·Chunyuan TangLushan Yu

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