LncRNA CSMD1-1 promotes the progression of Hepatocellular Carcinoma by activating MYC signaling.

Theranostics
Ji LiuHui-Yun Wang

Abstract

Emerging evidence suggests that long non-coding RNAs (lncRNA) play critical roles in the development and progression of diverse cancers including hepatocellular carcinoma (HCC), but the underlying molecular mechanisms of lncRNAs that are involved in hepatocarcinogenesis have not been fully explored. Methods: In this study, we profiled lncRNA expression in 127 pairs of HCC and nontumor liver tissues (a Discovery Cohort) using a custom microarray. The expression and clinical significance of lncCSMD1-1 were then validated with qRT-PCR and COX regression analysis in a Validation Cohort (n=260) and two External Validation Cohorts (n=92 and n=124, respectively). In vitro and in vivo assays were performed to explore the biological effects of lncCSMD1-1 on HCC cells. The interaction of lncCSMD1-1 with MYC was identified by RNA pull-down and RNA immunoprecipitation. The role of LncCSMD1-1 in the degradation of MYC protein was also investigated. Results: With microarray, we identified a highly upregulated lncRNA, lncCSMD1-1, which was associated with tumor progression and poor prognosis in the Discovery Cohort, and validated in another 3 HCC cohorts. Consistently, ectopic expression of lncCSMD1-1 notably promotes cell proliferation, migr...Continue Reading

Citations

Jan 26, 2021·OncoTargets and Therapy·Miao DaShuwen Han
Apr 4, 2021·International Journal of Molecular Sciences·Lok-Sze Wong, Chun-Ming Wong
Aug 3, 2021·Frontiers in Oncology·Pin ZhaoShaojun Xing

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Methods Mentioned

BETA
transgenic
ubiquitination
PCR
protein assay
Fluorescence
pull-down
Protein Extraction
electrophoresis
immunoprecipitation
xenograft

Software Mentioned

SAM analysis ( GSEA )
Image J
R
boot
GSEA
ImageJ
GraphPad Prism
Gmisc

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