LncRNA DBH-AS1 facilitates the tumorigenesis of hepatocellular carcinoma by targeting miR-138 via FAK/Src/ERK pathway

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Jie BaoYun Xu

Abstract

It has been reported that dysregulated lncRNAs are associated with the pathogenesis of human tumors including hepatocellular carcinoma (HCC). LncRNA DBH-AS1 was reported to be an oncogene in HCC. However, the molecular mechanisms of DBH-AS1 in HCC progression are largely undefined. The expressions of DBH-AS1 and miR-138 in HCC tissues and cells were examined by qRT-PCR. The effects of DBH-AS1 and miR-138 on cell viability, colony formation, apoptosis, and FAK/Src/ERK signaling pathway were determined by CCK-8, colony formation, flow cytometry, and western blot analyses, respectively. Luciferase reporter assay and RNA immunoprecipitation (RIP) assay were used to explore the interaction between miR-138 and DBH-AS1. Tumor xenograft assay was performed to confirm the function and mechanism of DBH-AS1 in the progression of HCC in vivo. DBH-AS1 expression was upregulated and miR-138 expression was downregulated in HCC tissues and cells. DBH-AS1 silencing and miR-138 overexpression reduced cell viability, inhibited colony formation, and induced apoptosis. Moreover, DBH-AS1 acted as a molecular sponge of miR-138 to downregulate miR-138 expression. Also, DBH-AS1 overexpression attenuated miR-138-mediated anti-proliferation and pro-apopt...Continue Reading

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Citations

Apr 7, 2020·Journal of Clinical Laboratory Analysis·Jinming Han, Xiaohan Shen
Jul 26, 2019·Analytical Cellular Pathology (Amsterdam)·Haihong ShiXia Hou
Jan 16, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Enjun LvWenjie Huang
Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lei ZhangKangsheng Tu
Mar 27, 2021·Frontiers in Cell and Developmental Biology·Soudeh Ghafouri-FardMohammad Taheri
Apr 4, 2021·International Journal of Molecular Sciences·Princess D RodriguezSeth Frietze
May 11, 2021·Frontiers in Oncology·Soudeh Ghafouri-FardMohammad Taheri
Jul 30, 2021·Seminars in Cancer Biology·Ganji Purnachandra NagarajuBassel F El-Rayes
Aug 10, 2021·Molecular Therapy : the Journal of the American Society of Gene Therapy·Xiaoting LinFeng Ye

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