LncRNA DGCR5 suppresses neuronal apoptosis to improve acute spinal cord injury through targeting PRDM5

Cell Cycle
Huafeng ZhangLei Xia

Abstract

Spinal cord injury (SCI) usually results in neurological damage. DGCR5 is closely related to neurological disorders, and this study aims to explore its role in neuronal apoptosis in acute SCI. The ASCI model was established in rats, and the Basso, Beattie, and Bresnahan (BBB) scoring was used to assess the neurological function. The expression of RNA and protein was quantified by quantitative real-time PCR (qRT-PCR) and western blotting, respectively. The oxygenglucose deprivation (OGD) was performed upon neurons and apoptosis was evaluated by flow cytometry. The interaction and binding between DGCR5 and PRDM5 was detected with RNA pull-down and RIP assay, respectively. DGCR5 was down-regulated in ASCI model rat and in neurons treated with hypoxia. Over-expression of DGCR5 inhibited neuronal apoptosis. Interaction between DGCR5 negatively regulated PRDM5 protein expression by binding and interacting with it. DGCR5 inhibited neuronal apoptosis through PRDM5. Over-expressed DGCR5 ameliorated ASCI in rat. DGCR5 suppresses neuronal apoptosis through directly binding and negatively regulating PRDM5, and thereby ameliorating ASCI.

References

Jul 20, 2007·Molecular and Cellular Biology·Zhijun DuanMarshall S Horwitz
Dec 4, 2008·RNA·Rory JohnsonLeonard Lipovich
Dec 29, 2011·Neurobiology of Disease·Rory Johnson
Jun 18, 2014·Journal of Neurotrauma·Amy L Bowes, Ping K Yip
Aug 13, 2014·Trends in Immunology·James A Heward, Mark A Lindsay
Apr 11, 2015·Nature Communications·Kun WangPei-Feng Li
Jun 23, 2015·Journal of Spinal Disorders & Techniques·Christopher D Witiw, Michael G Fehlings
Nov 13, 2015·Orphanet Journal of Rare Diseases·Louise F PorterGraeme C Black
Dec 23, 2015·Journal of Genetics·Mina KazemzadehAyla Valinezhad Orang
Apr 1, 2016·Cornea·Shazia MichealAnneke I den Hollander
Oct 1, 2016·BioMed Research International·Ya DingJinbo Liu
Nov 11, 2016·Neurochemical Research·Jie LiuFeng Zhang
Nov 30, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Ruyi HuangBeicheng Sun
May 30, 2017·Biochemical and Biophysical Research Communications·Weiqi LingJinbo Liu
Oct 17, 2017·Journal of Cellular Physiology·Hui-Xing DongJing Pan

❮ Previous
Next ❯

Citations

Jan 5, 2020·Cold Spring Harbor Symposia on Quantitative Biology·Hadas HezroniIgor Ulitsky
Jul 19, 2019·Frontiers in Molecular Neuroscience·Chun Yao, Bin Yu
Dec 12, 2019·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Jinhua DaiGuofang Chen
May 26, 2021·Journal of Neuroscience Research·Xiaoping TanShuyan Cong
Jun 22, 2021·Neurochemistry International·Mengqi ZhangKe-Jie Yin
Jul 30, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Haoming XiaYunfu Cui
Jul 30, 2021·Journal of Orthopaedic Surgery·Yiguang Zhou, Feng Yu
Feb 8, 2022·Cellular and Molecular Life Sciences : CMLS·Xizi WuJia Qian Wu

❮ Previous
Next ❯

Datasets Mentioned

BETA
AB051434

Methods Mentioned

BETA
protein assay
flow cytometry
pull-down
immunoprecipitation
transfection
RIP

Software Mentioned

Flow Jo
SPSS
vtro

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Blood Brain Barrier Regulation in Health & Disease

The blood brain barrier is essential in regulating the movement of molecules and substances in and out of the brain. Disruption to the blood brain barrier and changes in permeability allow pathogens and inflammatory molecules to cross the barrier and may play a part in the pathogenesis of neurodegenerative disorders. Here is the latest research in this field.

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.