LncRNA DICER1-AS1 promotes the proliferation, invasion and autophagy of osteosarcoma cells via miR-30b/ATG5.

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Zenghui GuCheng Zhang

Abstract

Osteosarcoma is a prevalent primary malignant tumor and long non-coding RNAs (lncRNAs) have been validated to modulate the osteosarcoma tumorigenesis. In present study, our research team investigates the role of a novel identified lncRNA DICER1-AS1 on the tumor progression and autophagy. Results showed that lncRNA DICER1-AS1 was up-regulated in osteosarcoma cells using microarray analysis and RT-PCR. Cellular functional experiments revealed that DICER1-AS1 knockdown suppressed the proliferation, migration, invasion and autophagy of osteosarcoma cells in vitro. Besides, DICER1-AS1 knockdown inhibited the protein expression levels of ATG5, LC3-II and Beclin 1, suggesting the inhibition on the autophagy of osteosarcoma cells. Moreover, miR-30b was verified to target 3'-UTR of DICER1-AS1 and ATG5 using bioinformatics tools and luciferase reporter assay or RNA-immunoprecipitation (RIP). Western blot showed that ATG5 protein expression was decreased in DICER1-AS1 knockdown and miR-30b mimics transfected cells, while increased in miR-30b inhibitor transfected cells, presenting a negative correlation with miR-30b and a positive correlation with DICER1-AS1. Finally, xenograft assay in vivo indicated that DICER1-AS1 knockdown inhibited t...Continue Reading

References

Jun 13, 2017·Biochemical and Biophysical Research Communications·Jingyi SongXuexiao Ma
Jul 1, 2017·The American Journal of Chinese Medicine·Yue-Ying Li, Yong-Hua Zhao
Jul 12, 2017·International Journal of Molecular Sciences·Svetlana Bortnik, Sharon M Gorski
Jul 18, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Zhihong LiShasha He
Aug 5, 2017·International Journal of Molecular Sciences·Yunho JinYonggeun Hong
Aug 9, 2017·Frontiers in Molecular Neuroscience·Shu YuanZi-Lin Li
Sep 4, 2017·Journal of Cellular and Molecular Medicine·Lijun CaiHuilin Yang
Oct 24, 2017·Cellular Signalling·Fatemeh Momen-Heravi, Shashi Bala
Oct 27, 2017·Frontiers in Pharmacology·Amal Kamal Abdel-AzizMohamed Elgendy

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Citations

Apr 6, 2019·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·G M VieraM S Brassesco
Aug 24, 2018·Journal of Hematology & Oncology·Hui SunMi-Die Xu
Jul 3, 2020·Journal of Molecular Neuroscience : MN·Amin SafaSoudeh Ghafouri-Fard
Jan 19, 2019·Genes·Sorina DinescuMarieta Costache
May 31, 2019·Human Genetics·Shi Q WongLouis R Lapierre
Apr 2, 2020·IUBMB Life·Zeinab JamaliHamed Mirzaei
May 8, 2020·Cardiology Research and Practice·Yexin ZhangBo Yan
Sep 22, 2019·Ecotoxicology and Environmental Safety·Yujiao ChenAi Gao
Jan 13, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Taheri
Feb 3, 2021·Autophagy·Soudeh Ghafouri-FardMohammad Taheri
Oct 24, 2020·Scientific Reports·Amin SafaSoudeh Ghafouri-Fard
Feb 1, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Yunus Akkoc, Devrim Gozuacik
Dec 15, 2019·European Journal of Pharmacology·Nader EbadiMir Davood Omrani
Jun 4, 2020·Aging and Disease·Cristina-Sorina CatanaIoana Berindan-Neagoe
Oct 15, 2021·Frontiers in Cell and Developmental Biology·Zhen TangXin Xiao
Aug 19, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jian DaiXiaoming Tang

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