LncRNA DIGIT Accelerates Tube Formation of Vascular Endothelial Cells by Sponging miR-134.

International Heart Journal
Chaofeng MiaoJiaxiang Wang

Abstract

Atherosclerosis is one of the most prevalent and important cardiac diseases, involving the heart and brain. This study aimed to explore the impacts of lncRNA Divergent to GSC induced by TGF-b family signaling (DIGIT) on vascular endothelial cells tube-formation capacity so as to reveal the potentials of DIGIT in atherosclerosis therapy. DIGIT expression in human microvascular endothelial HMEC-1 cells was silenced by transfection with shRNAs-targeted DIGIT. The effects of DIGIT silence on cell viability, migration, apoptosis, and tube formation were then assessed. Additionally, the cross-regulation between DIGIT and miR-134, and between miR-134 and Bmi-1 was detected to further reveal through which mechanism (s) DIGIT mediated HMEC-1 cells. The results showed that DIGIT silence significantly reduced cell viability, migration, tube-like structures formation, and induced apoptosis in HMEC-1 cells. DIGIT worked as a sponge for miR-134, and the anti-growth, anti-migratory, and anti-tube-formation functions of DIGIT silence on HMEC-1 cells were abolished by miR-134 suppression. Bmi-1 was a target of miR-134, and Bmi-1 upregulation abolished miR-134 overexpression-diminished cell growth, migration, and tube formation of HMEC-1 cells. ...Continue Reading

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Citations

Sep 5, 2020·Journal of Cellular and Molecular Medicine·Mengdie ChengHui Xin
Nov 20, 2019·Heart Failure Reviews·Qi JinZhihong Liu
Feb 6, 2020·Clinical and Translational Medicine·Estanislao NavarroMiguel Hueso
Aug 13, 2020·Journal of Cellular and Molecular Medicine·Nakwon ChoeDuk-Hwa Kwon
Sep 29, 2020·Drug Design, Development and Therapy·Kui ChiFeng Zhang
May 11, 2021·Frontiers in Cell and Developmental Biology·Xiangfu MengDonghui Zhang

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