lncRNA DLGAP1-AS2 Knockdown Inhibits Hepatocellular Carcinoma Cell Migration and Invasion by Regulating miR-154-5p Methylation.

BioMed Research International
Kai ChenXuejun Zhang

Abstract

DLGAP1-AS2 has been characterized as an oncogenic lncRNA in glioma. Our preliminary microarray analysis revealed the altered expression of DLGAP1-AS2 in hepatocellular carcinoma (HCC), but the role of DLGAP1-AS2 in HCC remains unknown. Expression of DLGAP1-AS2 and miR-154-5p in paired HCC and nontumor tissues from 62 HCC patients was determined by RT-qPCR. The 62 HCC patients were followed up for 5 years to analyze the prognostic value of DLGAP1-AS2 for HCC. DLGAP1-AS2 knockdown and miR-154-5p overexpression was achieved in HCC cells to study the relationship between them. Methylation of miR-154-5p was analyzed by methylation-specific PCR. Cell proliferation was analyzed by CCK-8 assay. DLGAP1-AS2 was upregulated in HCC and predicted poor survival. miR-154-5p was downregulated in HCC and inversely correlated with DLGAP1-AS2. In HCC cells, DLGAP1-AS2 knockdown resulted in the upregulation of miR-154-5p expression and decreased methylation of miR-154-5p gene. Transwell assay showed that DLGAP1-AS2 knockdown and miR-154-5p overexpression inhibited cell invasion and migration, and the combination of LGAP1-AS2 knockdown and miR-154-5p overexpression showed stronger effects. DLGAP1-AS2 knockdown may inhibit HCC cell migration and inv...Continue Reading

References

Oct 28, 2004·Gastroenterology·Timothy R MorganM Mazen Jamal
Oct 28, 2004·Gastroenterology·Stephen H CaldwellAbdullah M S Al-Osaimi
Sep 30, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Josep M Llovet, Jordi Bruix
Jan 27, 2010·World Journal of Gastroenterology : WJG·Piotr StefaniukAlicja Wiercinska-Drapalo
Dec 3, 2010·The Journal of Pathology·Thalia A FaraziThomas Tuschl
Jul 31, 2013·Current Pharmaceutical Design·Liang RongruiJiang Shiwen
Aug 28, 2014·Biochimica Et Biophysica Acta·Guodong YangLijun Yuan
Oct 27, 2016·Liver Cancer·Daniel Wai-Hung HoIrene Oi-Lin Ng
Sep 20, 2018·Liver International : Official Journal of the International Association for the Study of the Liver·Michael C WallaceGary P Jeffrey
Jan 4, 2020·Journal of Biochemistry·Wei MiaoHuilin Cheng
Jan 9, 2020·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Apr 23, 2020·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Katherine A McGlynnHashem B El-Serag

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
PCR
PCRs
transfections

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.