LncRNA DLX6-AS1 Contributes to Epithelial-Mesenchymal Transition and Cisplatin Resistance in Triple-negative Breast Cancer via Modulating Mir-199b-5p/Paxillin Axis.

Cell Transplantation
Chuang DuJingruo Li

Abstract

Triple-negative breast cancer (TNBC) is one of the most aggressive cancer types with high recurrence, metastasis, and drug resistance. Recent studies report that long noncoding RNAs (lncRNAs)-mediated competing endogenous RNAs (ceRNA) play an important role in tumorigenesis and drug resistance of TNBC. Although elevated lncRNA DLX6 antisense RNA 1 (DLX6-AS1) has been observed to promote carcinogenesis in various cancers, the role in TNBC remained unclear. In this study, expression levels of DLX6-AS1 were increased in TNBC tissues and cell lines when compared with normal tissues or breast fibroblast cells which were determined by quantitative real-time PCR (RT-qPCR). Then, CCK-8 assay, cell colony formation assay and western blot were performed in CAL-51 cells transfected with siRNAs of DLX6-AS1 or MDA-MB-231 cells transfected with DLX6-AS1 over expression plasmids. Knock down of DLX6-AS1 inhibited cell proliferation, epithelial-mesenchymal transition (EMT), decreased expression levels of BCL2 apoptosis regulator (Bcl-2), Snail family transcriptional repressor 1 (Snail) as well as N-cadherin and decreased expression levels of cleaved caspase-3, γ-catenin as well as E-cadherin, while up regulation of DLX6-AS1 had the opposite eff...Continue Reading

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Citations

Jan 26, 2021·OncoTargets and Therapy·Miao DaShuwen Han
Feb 13, 2021·Cancer Management and Research·Liqiang QiSu Lu
Feb 26, 2021·Pathology, Research and Practice·Soudeh Ghafouri-FardMohammad Taheri
Aug 16, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Ganesh Kumar BarikBhargab Kalita
Sep 1, 2021·Cancer Cell International·Biao LiuJianye Liu
Sep 12, 2021·Human Cell·Yalan LuoHailong Chen

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Methods Mentioned

BETA
electrophoresis
transfection
Immunoprecipitation
Pull-down
Assay
immunoprecipitation assay
xenografts

Software Mentioned

Targetscan
SPSS
Image J

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