LncRNA HOTAIR influences cell growth, migration, invasion, and apoptosis via the miR-20a-5p/HMGA2 axis in breast cancer

Cancer Medicine
Wenyan ZhaoMing Sun

Abstract

To study the regulatory effect of lncRNA HOTAIR/miR-20a-5p/HMGA2 axis on breast cancer (BC) cell growth, cell mobility, invasiveness, and apoptosis. The microarray data of lncRNAs and mRNAs with differential expression in BC tissues were analyzed in the Cancer Genome Atlas (TCGA) database. LncRNA HOX transcript antisense RNA (lncRNA HOTAIR) expression in BC was assessed by qRT-PCR. Cell viability was confirmed using MTT and colony formation assay. Cell apoptosis was analyzed by TdT-mediated dUTP nick-end labeling (TUNEL) assay. Cell mobility and invasiveness were testified by transwell assay. RNA pull-down and dual luciferase assay were used for analysis of the correlation between lncRNA HOTAIR and miR-20a-5p, as well as relationship of miR-20a-5p with high mobility group AT-hook 2 (HMGA2). Tumor xenograft study was applied to confirm the correlation of lncRNA HOTAIR/miR-20a-5p/HMGA2 axis on BC development in vivo. The expression levels of the lncRNA HOTAIR were upregulated in BC tissues and cells. Knockdown lncRNA HOTAIR inhibited cell propagation and metastasis and facilitated cell apoptosis. MiR-20a-5p was a target of lncRNA HOTAIR and had a negative correlation with lncRNA HOTAIR. MiR-20a-5p overexpression in BC suppressed ...Continue Reading

References

May 5, 2007·Molecular Carcinogenesis·Britta MeyerJörn Bullerdiek
Nov 13, 2007·Advances in Experimental Medicine and Biology·Ana Maria Gonzalez-AnguloGabriel N Hortobagyi
Nov 16, 2007·Nature Reviews. Cancer·Alfredo Fusco, Monica Fedele
Aug 5, 2008·Nature Immunology·Noam Stern-GinossarOfer Mandelboim
Aug 13, 2008·The Journal of Cell Biology·Zuoren YuRichard G Pestell
Apr 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Zuoren YuRichard G Pestell
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Jan 20, 2012·Cancer Research·Takeshi NiinumaYasuhisa Shinomura
Feb 1, 2012·The Journal of International Medical Research·Y J GengG Y Wang
Nov 22, 2013·Breast Cancer Research and Treatment·Kristina P SørensenTorben A Kruse
Mar 13, 2014·The Journal of Biological Chemistry·Takeshi ChiyomaruSoichiro Yamamura
Oct 23, 2014·Development·Sowmya ParameswaranIqbal Ahmad
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
May 1, 2015·Journal of Translational Medicine·Sifeng TaoQiang Chen
May 29, 2015·RNA Biology·Zhen XingLiuqing Yang
Aug 19, 2015·Current Gene Therapy·Oleg ShuvalovNickolai A Barlev
Jan 11, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Gillian BrowneJane B Lian
Jul 5, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yuzhou WangYing Zhang

❮ Previous
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Citations

Sep 5, 2019·Cells·Yujing ChiJichun Yang
Sep 1, 2019·Current Pharmaceutical Design·Asma VafadarHamed Mirzaei
Jan 25, 2020·Journal of Cellular and Molecular Medicine·Ping LiXuelei Zhang
Dec 5, 2019·Cancer Investigation·Sheng-Hong ShiZhi-Gang Zhuang
Feb 28, 2020·Experimental and Therapeutic Medicine·Zheng XiaoXi Zeng
Jan 12, 2020·Science China. Life Sciences·Chengcheng ZhangYin Wang
Jul 29, 2020·Journal of Biochemical and Molecular Toxicology·Yun ZhouShan-Shan Wang
Apr 5, 2020·Journal of Translational Medicine·Hossein MozdaraniRoghayeh Rahbar Parvaneh
Jun 25, 2020·Cancer Metastasis Reviews·Belinda J Petri, Carolyn M Klinge
May 29, 2018·Environmental Science and Pollution Research International·Zhibin ZuoQibao Wang
Jun 15, 2019·Journal of Experimental & Clinical Cancer Research : CR·Xue GaoLianhong Li
Sep 16, 2020·Cell Death & Disease·Lingxia LiuJun Lu
Jul 1, 2020·Journal of Cellular and Molecular Medicine·Guangcheng DaiBoxin Xue
Sep 15, 2020·Cancer Management and Research·Zhuo Wang, Ruqing Xu
Jan 17, 2020·Journal of Cellular Biochemistry·Chunjue YuanYunbao Pan
Mar 28, 2019·Bone & Joint Research·J ZhangF Guo
Apr 13, 2019·Cellular & Molecular Biology Letters·Liang SunShu Meng
Jul 23, 2020·Cancer Cell International·Yuanyuan WangShaohong Wang
Nov 3, 2020·DNA and Cell Biology·Liyu QianBing Yang
Dec 22, 2020·Frontiers in Cell and Developmental Biology·Xin WangDong Zhao
Jan 5, 2021·Frontiers in Oncology·Blanca I Quiñones-DíazPablo E Vivas-Mejía
Nov 26, 2020·Experimental and Molecular Pathology·Soudeh Ghafouri-FardMohammad Taheri
Jan 7, 2020·Clinica Chimica Acta; International Journal of Clinical Chemistry·Taruna RajagopalNageswara Rao Dunna
Oct 27, 2020·Cancer Biomarkers : Section a of Disease Markers·Dongdi WuBiao Wu
Nov 1, 2020·Cells·Christian T StackhouseChristopher D Willey
Dec 30, 2020·International Journal of Molecular Sciences·Miguel Ángel Cáceres-DuránAmanda Ferreira Vidal

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Methods Mentioned

BETA
transfection
PCR
pull down
MDA
transfecting

Software Mentioned

TargetScan
miRcode
GraphPad Prism
GraphPad

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