LncRNA HOTAIR/miR-613/c-met axis modulated epithelial-mesenchymal transition of retinoblastoma cells

Journal of Cellular and Molecular Medicine
Ge YangQiuming Li

Abstract

Since lncRNAs could modulate neoplastic development by modulating downstream miRNAs and genes, this study was carried out to figure out the synthetic contribution of HOTAIR, miR-613 and c-met to viability, apoptosis and proliferation of retinoblastoma cells. Totally 276 retinoblastoma tissues and tumour-adjacent tissues were collected, and human retinoblastoma cell lines (ie, Y79, HXO-Rb44, SO-Rb50 and WERI-RB1) were also gathered. Moreover, transfections of pcDNA3.1-HOTAIR, si-HOTAIR, miR-613 mimic, miR-613 inhibitor, pcDNA3.1/c-met were performed to evaluate the influence of HOTAIR, miR-613 and c-met on viability, apoptosis and epithelial-mesenchymal transition (EMT) of retinoblastoma cells. Dual-luciferase reporter gene assay was also arranged to confirm the targeted relationship between HOTAIR and miR-613, as well as between miR-613 and c-met. Consequently, up-regulated HOTAIR and down-regulated miR-613 expressions displayed associations with poor survival status of retinoblastoma patients (P < 0.05). Besides, inhibited HOTAIR and promoted miR-613 elevated E-cadherin expression, yet decreased Snail and Vimentin expressions (P < 0.05). Simultaneously, cell proliferation and cell viability were also less-motivated (P < 0.05)....Continue Reading

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Apr 18, 2019·Current Eye Research·Xueman LyuLina Wang
May 1, 2019·Journal of Cellular Biochemistry·Lufei WangHui Zhang
Jun 4, 2020·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Yu XuYuqing Xiong
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Methods Mentioned

BETA
chip
chips
Feature Extraction
transfection
Assay
flow cytometry

Software Mentioned

SPSS
Agilent Feature Extraction
Starbase
Gene Spring
Primer Express

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