LncRNA IMFNCR Promotes Intramuscular Adipocyte Differentiation by Sponging miR-128-3p and miR-27b-3p

Frontiers in Genetics
Meng ZhangGuiRong Sun

Abstract

Poultry meat quality is affected by many factors, among which intramuscular fat (IMF) is predominant. IMF content affects the tenderness, juiciness, and flavor of chicken. An increasing number of studies are focusing on the functions of lncRNAs in adipocyte differentiation. However, little is known about lncRNAs associated with intramuscular adipocyte differentiation. In the present study, we focused on an up-regulated lncRNA during intramuscular adipogenetic differentiation, which we named intramuscular fat-associated long non-coding RNA (IMFNCR). IMFNCR promotes intramuscular adipocyte differentiation. In-depth analyses showed that IMFNCR acts as a molecular sponge for miR-128-3p and miR-27b-3p and that PPARG is a direct target of miR-128-3p and miR-27b-3p in chicken. High-fat and high-protein diet inhibited chicken IMFNCR level in vivo. Moreover, IMFNCR level was positively correlated with PPARG mRNA level in chicken breast muscle tissues, a vital corollary to ceRNA function. Altogether, our research showed that IMFNCR acts as a ceRNA to sequester miR-128-3p and miR-27b-3p, leading to heightened PPARG expression, and thus promotes intramuscular adipocyte differentiation. Taken together, our findings may contribute to a more ...Continue Reading

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Citations

Nov 14, 2019·International Journal of Molecular Sciences·Xiaopei ZhangFafu Shen
Nov 27, 2020·Journal of Cellular Physiology·Qi ShangHui Ren
Mar 18, 2021·Molecular and Cellular Biochemistry·Zhaoxiong LeiYun Ma
Nov 6, 2021·British Poultry Science·Tao ZhangJinyu Wang

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Datasets Mentioned

BETA
PRJNA380024

Methods Mentioned

BETA
PCR
transfection
Fluorescence

Software Mentioned

CPC ( Coding Potential Calculator )
IMFNCR
SPSS
Coding Potential Calculator ( CPC )

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