LncRNA MALAT-1 competitively regulates miR-124 to promote EMT and development of non-small-cell lung cancer

Anti-cancer Drugs
Jiasheng WuLin Cai

Abstract

This study was carried out to explore the mechanism of LncRNA MALAT-1 as a competing endogenous RNA to regulate miR-124 in epithelial-mesenchymal transition and development of non-small-cell lung cancer (NSCLC). NSCLC and adjacent tissues were collected for RT-qPCR. The correlation of MALAT-1 and miR-124 was analyzed by Pearson. MALAT-1 expression was measured in NSCLC A549, NCI-H460, NCI-H529, SK-MES-1 cells, and 16HBE cells. A549 cells were selected for cell transfection experiments after the creation of six groups. Luciferase reporter assay and RNA immunoprecipitation were used to verify the relationship between MALAT-1 and miR-124. Expressions of E-cadherin and vimentin were determined by western blot. Cell variability, apoptosis, invasion, and migration were measured by MTT, FCM, transwell assay, and scratch test. LncRNA MALAT-1 expression was higher in NSCLC tissues than that in adjacent tissues, but a lower expression of miR-124 was detected in the former tissues than in the latter tissues. Compared with 16HBE cells, MALAT-1 was highly expressed in NSCLC tissues. Compared with the blank group, E-cadherin and cell apoptosis were increased, but vimentin, cell variability, cell invasion, and migration ability in the si-MALA...Continue Reading

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Citations

Apr 27, 2019·Journal of Cellular and Molecular Medicine·Yigong CuiXuehui Wu
May 2, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Fei LuoYan Wang
Dec 15, 2019·Journal of Clinical Laboratory Analysis·Qining JiangZhengyong Luo
Jul 16, 2019·Cell & Bioscience·Ying ZhangLiu Yang
Oct 30, 2020·Cancer Management and Research·Shijian FuQuanzhong Liu
Apr 13, 2021·Frontiers in Molecular Biosciences·Bashdar Mahmud HussenSoudeh Ghafouri-Fard
Jun 3, 2021·International Journal of Molecular Sciences·Rikako Sanuki, Tomonori Yamamura
Jul 3, 2021·International Journal of Molecular Sciences·Sonia TerriacaAugusto Orlandi
Oct 3, 2021·Journal of Cellular and Molecular Medicine·Jin-Liang Yue, Shu-Feng Zheng
Nov 17, 2021·Critical Reviews in Food Science and Nutrition·Mina HomayoonfalBahman Yousefi

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Methods Mentioned

BETA
transfection
Assay
RIP
PCR
electrophoresis
immunoprecipitation
flow cytometry

Software Mentioned

Image J
Instrument Vision Assistant
SPSS
National
OpticonMonitor3

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