LncRNA MALAT1 is Neuroprotective in a Rat Model of Spinal Cord Ischemia-Reperfusion Injury Through miR-204 Regulation

Current Neurovascular Research
Yong QiaoXiuwen Wang

Abstract

This study was to investigate the neuroprotective effect of long noncoding RNAs Metastasis associated lung adenocarcinoma transcript-1 (lncRNA MALAT-1) in Spinal Cord Ischemic/Reperfusion Injury (SCIRI). Quantitative real-time PCR (RT-qPCR) was used to examine the expressions of MALAT1, miR-204 and Bcl-2, while western blot was performed to examine Bcl-2. Besides, apoptosis was evaluated by the percentage of cell viability and apoptotic cells. Neurological evaluation was performed by measuring hindlimb locomotor function. The expression of MALAT1 and Bcl-2 was decreased, while miRNA-204 (miR-204) was up-regulated in rats SCIRI model and neurocyte lines under hypoxic conditions. Oxygen, Glucose Deprivation (OGD) promoted apoptosis of neurocytes, downregulated MALAT1 and Bcl-2 and elevated miR-204 expression, however, overexpression of MALAT1 notably reversed this trend. Nevertheless, knockdown of MALAT1 increased cell apoptosis, decreased MALAT1 and Bcl-2 but upregulated miR-204. MALAT1 overexpression-induced anti-apoptosis and knockdowninduced pro-apoptosis were obviously reversed by synchronously overexpression and knockdown of miR-204, respectively. MALAT1-treated SCIRI rats exhibited lower Motor Deficit Index (MDI) scores, h...Continue Reading

Citations

May 30, 2019·Journal of Cellular and Molecular Medicine·Zheng LiXiaodong Liu
Apr 30, 2020·Stem Cells·Baoyan FanXian Shuang Liu
Aug 23, 2019·Molecular Therapy. Nucleic Acids·Li Zhang, Handong Wang
Dec 11, 2020·BioMed Research International·Chengwu GongJichun Liu
Apr 18, 2021·Journal of Orthopaedic Surgery and Research·Chongcheng GongYang Shen
May 7, 2021·Regenerative Medicine·Xu-Dong GuoXue-Wen Kang
May 16, 2021·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Wenzhao WangWei Xie
Jul 10, 2021·Journal of Clinical Laboratory Analysis·Hai-Yuan LuHai-Tao Jiang
Jun 15, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Chao-Ming DaHai-Hong Zhang

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