PMID: 32521885Jun 12, 2020Paper

lncRNA MALAT1 regulates the expression level of miR-21 and interferes with the biological behavior of colon cancer cells

Journal of B.U.ON. : Official Journal of the Balkan Union of Oncology
Bin HuangYan Li

Abstract

This study aimed to verify whether the regulation of miR-21 expression by lncRNA MALAT1 interferes with the biological behavior and mechanism of colon cancer cells. RT-qPCR was used to detect the expression of MALAT1 in colon cancer and paracancerous tissues and different colon cancer cell lines (HT-29, SW480, SW620, CaCo-2). The relationship between MALAT1 and clinicopathological parameters of colon cancer patients and the interaction of MALAT1 and miR-21 by dual luciferase reporter gene detection were detected. Transwell invasion assay detected the invasive ability of colon cancer cells after MALAT1 inhibition and scratch assay detected the migration ability of colon cancer cells after MALAT1 inhibition. Subcutaneous tumor formation was detected in nude mice to measure the inhibition of the tumor size and volume of MALAT1 colon cancer cells. Compared with paracancerous tissues, MALAT1 expression was significantly increased in colon cancer tissues. MALAT1 expression was the highest in HT-29 colon cancer cell line. MALAT1 was specifically bound to miR-21 3' UTR. Inhibition of MALAT1 could inhibit colon cancer cell invasion and migration ability, and tumor formation in nude mice showed that the tumor volume and weight of the tum...Continue Reading

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