LncRNA MALAT1 silencing protects against cerebral ischemia-reperfusion injury through miR-145 to regulate AQP4

Journal of Biomedical Science
Hongwei WangWei Chen

Abstract

The present study aimed to verify whether long noncoding RNA (lncRNA) MALAT1 is involved in brain tissue damage induced by ischemia-reperfusion injury, and to explore the mechanism by which MALAT1 regulates aquaporin 4 (AQP4). In this study, we established glucose deprivation (OGD)/reoxygenation (RX) astrocyte cell model and middle cerebral artery occlusion (MCAO)/reperfusion mouse model in vitro and in vivo. Then cell counting kit-8 assay, flow cytometry analysis, Triphenyltetrazolium chloride (TTC) staining, and western blotting were used to determine cell viability, cell apoptosis, cerebral infarction volume, and the abundance of AQP4, respectively. We found that the level of MALAT1 was significantly upregulated in both the MCAO/reperfusion model and OGD/RX model. Knockdown of MALAT1 increased cell viability and reduced cell apoptosis in MA-C cells, while an AQP4 siRNA combined with a siRNA targeting MALAT1 could not enhance this effect. Further experiments showed that MALAT1 positively regulated AQP4 expression via miR-145. The MALAT1 siRNA did not alleviate the exacerbation of damage after miR-145 inhibitor action. However, an miR-145 inhibitor reversed the protection effects of MALAT1, indicating that MALAT1 silencing pro...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jan 16, 2007·Brain Research Reviews·Suresh L MehtaRam Raghubir
May 18, 2007·Annual Review of Cell and Developmental Biology·Natascha Bushati, Stephen M Cohen
Jul 10, 2007·Journal of the Neurological Sciences·Jaishri O Blakeley, Rafael H Llinas
Sep 16, 2010·Physiological Genomics·Cameron Rink, Savita Khanna
Jan 24, 2013·International Journal of Molecular Sciences·Jun Rong TanKandiah Jeyaseelan
Mar 7, 2013·Nature Structural & Molecular Biology·Tim R Mercer, John S Mattick
Mar 27, 2013·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Tony GutschnerSven Diederichs
Oct 10, 2013·Nature Reviews. Molecular Cell Biology·Sarah Geisler, Jeff Coller
Feb 12, 2014·Journal of Basic and Clinical Physiology and Pharmacology·Antonio SiniscalchiGiovambattista De Sarro
Mar 8, 2014·Circulation Research·Katharina M MichalikStefanie Dimmeler
Apr 8, 2014·Neurochemistry International·Ke-Jie YinY Eugene Chen
Sep 27, 2014·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Jin XuShu-Juan Zhu
Feb 12, 2015·The New England Journal of Medicine·Bruce C V CampbellUNKNOWN EXTEND-IA Investigators
May 15, 2015·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Chen LiLongbang Chen
Feb 5, 2016·Biochemical and Biophysical Research Communications·Shao-Peng LinQiu-Jie Ma
Mar 5, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Jin FanGuoyong Yin
Mar 19, 2016·International Journal for Numerical Methods in Biomedical Engineering·Mohd Jamil Mohamed Mokhtarudin, Stephen J Payne
Sep 8, 2016·Annals of Emergency Medicine·Joseph B MillerChristopher Lewandowski
Nov 30, 2016·Journal of Translational Medicine·Quan ZhangPatrizia Farci
Jan 18, 2017·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xuejing ZhangKe-Jie Yin
Jan 31, 2017·Neurochemistry International·Raghavendar ChandranRaghu Vemuganti
May 24, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Yin XiangQianhui Li
Aug 31, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Dong GuoShaofeng Shui
Feb 17, 2018·Cell Death & Disease·Mei-Hua BaoZhong-Ping Feng
May 6, 2019·Metabolic Brain Disease·Masoumeh AlishahiSeyed Esmaeil Khoshnam
May 8, 2019·Biochemical and Biophysical Research Communications·Hai-Yun GaiDong Wang

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Citations

Jun 22, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Taheri
Mar 8, 2021·Genomics·Vishal Chavda, Kajal Madhwani
Jun 22, 2021·Neurochemistry International·Mengqi ZhangKe-Jie Yin
Jul 20, 2021·Neurochemistry International·Suresh L MehtaRaghu Vemuganti
Aug 13, 2021·Journal of Biochemical and Molecular Toxicology·Haijuan JingHongqi Lin

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Methods Mentioned

BETA
reverse transcription PCR
PCR
Assay
transfection
Flow cytometry

Software Mentioned

GraphPad Prism
Leica
GraphPad
Image Lab
StarBase

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