LncRNA MIAT facilitated BM-MSCs differentiation into endothelial cells and restored erectile dysfunction via targeting miR-200a in a rat model of erectile dysfunction

European Journal of Cell Biology
Hui WangLu Li

Abstract

Bone-marrow derived mesenchymal stem cells (BM-MSCs) implantation effectively restored rats' erectile dysfunction (ED). Long noncoding RNA (LncRNA)-myocardial infarction-associated transcript (MIAT) has been reported to play an important role in regulating endothelial cells (ECs) function via vascular endothelial growth factor (VEGF) that induced BM-MSCs differentiation into ECs. However, the molecular functions and biological roles of lncRNA MIAT in ED remained unclear. The rat model of ED was established. Quantitative real-time PCR (qRT-PCR) and western blotting were used to detect the expression of lncRNA MIAT, von Willebrand factor (vWF), vascular endothelial cadherin (VE-cadherin), endothelial NO synthase (eNOS) and VEGF following BM-MSCs transfection. Erectile function was evaluated by intra-cavernous pressure/mean artery pressure (ICP/MAP). Furthermore, RNA immunoprecipitation (RIP) assay and RNA pull down as well as luciferase reporter assay were carried out to examine the interaction among lncRNA MIAT, miR-200a and VEGF. BM-MSCs restored ED by upregulating lncRNA MIAT. LncRNA MIAT was upregulated in a time-dependent manner during BM-MSCs differentiation into ECs. LncRNA MIAT regulated VEGF via targeting miR-200a, there...Continue Reading

Citations

Apr 24, 2020·Circulation Research·Nicolas Jaé, Stefanie Dimmeler
May 23, 2020·Andrologia·Sixiang ChenKeyang Xu
Aug 25, 2020·DNA and Cell Biology·Shanfei GeYan-Qing Yu
Aug 19, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Wei HuoHai Li
Mar 22, 2019·Non-coding RNA·Diewertje I BinkReinier A Boon
Mar 28, 2020·Stem Cells International·Selma MaachaChiara Cugno
Apr 6, 2019·World Journal of Stem Cells·Zhong-Yu XieHui-Yong Shen
Dec 20, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shu-Lan QuYi-Fu Yang
Aug 10, 2021·Frontiers in Cell and Developmental Biology·Yanlei YangRobert Chunhua Zhao

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