LncRNA MIAT/miR-133a-3p axis regulates atrial fibrillation and atrial fibrillation-induced myocardial fibrosis

Molecular Biology Reports
Lixia YaoRuiqin Xie

Abstract

Atrial fibrillation (AF) is a commonly encountered heart arrhythmia and a risk factor for cardiovascular system. The purpose of the present study was to explore the role of long non-coding RNA myocardial infarction-associated transcript (MIAT) in AF and AF-induced myocardial fibrosis and the possible mechanisms involved in this process. We successfully induced an AF rat model. Expression of MIAT presented a dramatic increase, while microRNA (miR)-133a-3p presented a dramatic decrease in atrium tissues of rats with AF induction. In addition, we also found that MIAT was highly expressed and miR-133a-3p was significantly reduced in peripheral blood leukocyte of AF patients. For biological function exploration of MIAT/miR-133a-3p axis, MIAT was knocked down using small hairpin RNA (shRNA) lentivirus injection and the rescue experiments were performed simultaneously by inhibiting miR-133a-3p using anti-miR-133a-3p lentivirus injection in rats with AF. MIAT downregulation significantly alleviated AF, increased atrial effective refractory period (AERP), and reduced the duration of AF as well as cardiomyocytes apoptosis. Whereas these effects of MIAT downregulation on AF were reversed by anti-miR-133a-3p administration. Luciferase repo...Continue Reading

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Citations

Aug 6, 2020·International Journal of Molecular Sciences·Emre BektikDa-Zhi Wang
Aug 14, 2020·International Journal of Molecular Sciences·Estefanía Lozano-VelascoHouria Daimi
Jan 15, 2021·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Federica ReyStephana Carelli
Dec 15, 2020·Life Sciences·Savalan Babapoor-FarrokhranSumeet K Mainigi
Aug 17, 2021·Frontiers in Cardiovascular Medicine·Shengyuan HuangChengming Fan

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Methods Mentioned

BETA
PCR
Assay

Software Mentioned

GraphPad Prism
MIAT

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