lncRNA-mRNA competing endogenous RNA network in IR-hepG2 cells ameliorated by APBBR decreasing ROS levels: a systematic analysis

PeerJ
Min Lin, Zhu-Jun Mao

Abstract

Radix Astragali (Astragalus membranaceus var. mongholicus (Bunge)) and Coptis chinensis (Coptis chinensis var. angustiloba) are two commonly prescribed traditional Chinese herbs for diabetes. Astragalus Polysaccharide (AP) and Berberine (BBR) are active ingredients of these two herbs respectively and they are scientifically proved to have immunomodulatory and anti-inflammatory effects. They are also known for their antidiabetic potential by ameliorating insulin resistance (IR). AP and BBR have shown different advantages in treating diabetes according to previous reports. However, very few studies focus on the combined activities of the two potential antidiabetic ingredients. In this study, we discovered that reactive oxygen species (ROS) accumulated in IR-hepG2 cells and APBBR can decrease ROS level in model group significantly. We conjectured that APBBR can ameliorate IR in hepG2 cells by decreasing ROS level. In order to verify this hypothesis, we obtained phenotype and transcriptome information of IR-HepG2 cells and explore the underlying mechanism of the combination of AP and BBR(APBBR) activity on the relationship between ROS change in IR at whole-transcriptome level, so as to shed new light to efficacy and application of ...Continue Reading

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Citations

Jun 12, 2021·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Xuemei LiShujun Zhao
Jul 25, 2021·International Journal of Molecular Sciences·Vianet Argelia Tello-FloresEugenia Flores-Alfaro

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Methods Mentioned

BETA
RNA-seq
flow cytometry

Software Mentioned

StringTie
R package
TargetScan
Cutadapt
Miranda
GraphPad Prism
Cytoscape
Ensembl
R package Ballgown
scripts

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