LncRNA NEAT1 Promotes Deterioration of Hepatocellular Carcinoma Based on In Vitro Experiments, Data Mining, and RT-qPCR Analysis

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Zhi-An LingYi-Wu Dang

Abstract

Accumulated evidence indicates that lncRNA NEAT1 has important roles in various malignant tumors. In this study, we conducted a comprehensive analysis to explore the exact role of NEAT1 in hepatocellular carcinoma (HCC). The effects of NEAT1 on cell proliferation, apoptosis, migration, and invasion were measured by in vitro experiments. The expression level and clinical value of NEAT1 in HCC was evaluated based on data from The Cancer Genome Atlas (TCGA), Oncomine, and in-house real-time quantitative (RT-qPCR). Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, and protein-protein interaction (PPI) network analyses were conducted to investigate the potential molecular mechanisms of NEAT1. NEAT1 siRNA not only inhibited proliferation, migration, and invasion of HCC cells but also induced HCC cell apoptosis. A total of four records from TCGA, Oncomine, and RT-qPCR analysis were combined to assess the expression level of NEAT1 in HCC. The pooled standard mean deviation (SMD) indicated that NEAT1 was up-regulated in HCC (SMD = 0.54; 95% CI, 0.36-0.73; P < 0.0001). The area under the curve value of the summary receiver operating characteristic curve was 0.71. NEAT1 expression was also related to race (P = 0....Continue Reading

Citations

Jun 30, 2019·Expert Review of Molecular Diagnostics·Shinichi UmedaYasuhiro Kodera
Sep 5, 2019·Cells·Yujing ChiJichun Yang
May 1, 2019·Journal of Cellular Biochemistry·Lufei WangHui Zhang
Sep 3, 2020·Non-coding RNA·Shizuka Uchida, Sakari Kauppinen
Jun 30, 2019·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Danny Ka-Ho WongMan-Fung Yuen
Dec 29, 2020·Cancer Letters·Arun NandwaniMalabika Datta
Nov 24, 2020·Frontiers in Cell and Developmental Biology·Wei ZhouQifa Ye

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