LncRNA NEAT1 Regulates 5-Fu Sensitivity, Apoptosis and Invasion in Colorectal Cancer Through the MiR-150-5p/CPSF4 Axis

OncoTargets and Therapy
Xuesong WangMeishuang Li

Abstract

Colorectal cancer (CRC) is one of the most prevalent malignancies in the world. Long non-coding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) is involved in the development of many cancers. However, its role and mechanism in CRC progression still need further exploration. The expression levels of lnc-NEAT1, microRNA-150-5p (miR-150-5p) and cleavage and polyadenylation specific factor 4 (CPSF4) were determined by quantitative real-time PCR (qRT-PCR). The sensitivity of cells to 5-fluorouracil (5-Fu) was measured by 3-(4,5-dimethyl-2 thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. Cell apoptosis and invasion were evaluated by flow cytometry and transwell assays, respectively. Western blot (WB) analysis was used to assess the levels of resistance-related proteins and CPSF4 protein. Besides, dual-luciferase reporter assay was used to verify the interactions among lnc-NEAT1, miR-150-5p and CPSF4. Also, mice xenograft models were used to determine the effect of lnc-NEAT1 on CRC tumor growth in vivo. In CRC, the expression of lnc-NEAT1 was upregulated and miR-150-5p was downregulated, and the expression of both was negatively correlated. Silencing of lnc-NEAT1 promoted the 5-Fu sensitivity, enhanced the apop...Continue Reading

References

Apr 15, 2003·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Carolyn C Compton
Aug 2, 2011·Cell·Leonardo SalmenaPier Paolo Pandolfi
Nov 15, 2013·Lancet·Hermann BrennerChristian Peter Pox
Jan 17, 2014·Nature·Yvonne TayPier Paolo Pandolfi
Apr 26, 2014·World Journal of Gastroenterology : WJG·Xi WangXiao-Tong Hu
May 12, 2015·Cancer Cell International·Kaladhar B Reddy
Jan 26, 2016·CA: a Cancer Journal for Clinicians·Wanqing ChenJie He
Mar 12, 2016·Journal of the American College of Cardiology·Reinier A BoonStefanie Dimmeler
Mar 2, 2017·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Aug 10, 2017·Cell Proliferation·Sirong ShiYunfeng Lin
Mar 2, 2018·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Zongjuan LiLijuan Zou
Apr 11, 2019·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jianrong WuRanran Tang
Jul 14, 2019·Biochimica Et Biophysica Acta. Molecular Cell Research·Qian YangXiaojun Wu
Jul 16, 2019·Cancer Biomarkers : Section a of Disease Markers·Shui-Lan ZouZhi-Xin Kang
Jul 20, 2019·International Journal of Oncology·Yamei PangGanghua Yang
Jul 28, 2019·International Journal of Molecular Sciences·Xinping LiYi Pan

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Methods Mentioned

BETA
PCR
transfection
electrophoresis
Assay
Xenograft
Flow cytometry

Software Mentioned

SPSS
StarBase2
ARRIVE
TargetScan Human

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