lncRNA nuclear-enriched abundant transcript 1 promotes cell proliferation and invasion by targeting miR-186-5p/HIF-1α in osteosarcoma

Journal of Cellular Biochemistry
Hongyu Tan, Liang Zhao

Abstract

Increasing evidence shows that the long noncoding RNA nuclear enriched abundant transcript 1 (NEAT1) plays important roles in tumor progression. However, the function and the underlying mechanism of NEAT1 in osteosarcoma (OS) remain unclear. In the present study, we found that NEAT1 expression was significantly upregulated in OS tissues and cell lines. High NEAT1 expression was closely associated with advanced clinicopathologic features and poor overall survival of patients with OS. Using in vitro function assay, we found that NEAT1 could promote the proliferation, invasion, and epithelial-mesenchymal transition (EMT) process of OS cells. NEAT1 could also promote OS cell growth in vivo. In addition, our studies showed that miR-186-5p was a downstream target of NEAT1 in OS. Functionally, miR-186-5p suppressed the proliferation, invasion, and EMT process of OS cells. Furthermore, our data revealed that HIF-1α was a downstream target of miR-186-5p and that NEAT1 could exert its tumor oncogenic roles on OS cells via the miR-186-5p/HIF-1α axis. Taking our results together, we elucidated that the NEAT1/miR-186-5p/HIF-1α axis might be a therapeutic approach for the treatment of OS.

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Citations

Feb 5, 2020·Journal of Experimental & Clinical Cancer Research : CR·Xiafeng PengChaoying Liu
Jan 7, 2021·Cancers·Marek SamecPeter Kubatka
Jan 8, 2021·International Journal of General Medicine·Lili WangYongning Sun

Related Concepts

HIF1A protein, human
MIRN186 microRNA, human
NEAT1 long non-coding RNA, human
Metazoa
Malignant Bone Neoplasm
Cell Motility
Mice, Inbred BALB C
Mice, Nude
Neoplasm Invasiveness
Osteosarcoma