lncRNA-PDPK2P promotes hepatocellular carcinoma progression through the PDK1/AKT/Caspase 3 pathway

Molecular Oncology
Weidong PanSong Guo Zheng

Abstract

Hepatocellular carcinoma (HCC) is a malignancy with one of the worst prognoses. Long noncoding RNA (lncRNA) are emerging as an important regulator of gene expression and function, leading to the development of cancer. The aim of this study was to determine the relationship between lncRNA and HCC and to further guide clinical therapy. lncRNA in HCC and adjacent tissues were screened, and the correlation between lncRNA-PDPK2P expression in liver tissues and the pathological characteristics and severity of HCC was assessed. The effects of PDPK2P on HCC proliferation, apoptosis, metastasis, and invasion were also systematically investigated via CCK-8 assay, flow cytometry, scratch wound healing, and transwell assay, respectively. The relationship between PDPK2P and PDK1 was verified by RNA pull-down, rescue experiments and western blot. lncRNA-PDPK2P was highly expressed in HCC tissues with a distinct positive correlation between PDPK2P and PDK1, and the upregulation was clinically associated with a larger tumor embolus, low differentiation, and poor survival. Mechanistically, lncRNA-PDPK2P interacted with PDK1 and promoted HCC progression through the PDK1/AKT/caspase 3 signaling pathway. lncRNA-PDPK2P can promote HCC progression, ...Continue Reading

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Citations

Jan 25, 2020·Journal of Experimental & Clinical Cancer Research : CR·Xiu-Yan HuangZhao-You Tang
Apr 7, 2020·Journal of Clinical Laboratory Analysis·Jinming Han, Xiaohan Shen
Aug 31, 2019·Cancers·Saranya Chidambaranathan Reghupaty, Devanand Sarkar
Mar 19, 2020·Frontiers in Cell and Developmental Biology·Weiyang LouPeifen Fu
Mar 26, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Chen XueJunjun Jia
May 7, 2021·International Journal of Biological Macromolecules·Milad AshrafizadehMichael R Hamblin
May 11, 2021·Frontiers in Oncology·Soudeh Ghafouri-FardMohammad Taheri

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Methods Mentioned

BETA
immunoprecipitation
gene knockout
chips
feature extraction
PCR
flow cytometry
transfection
electrophoresis

Software Mentioned

Arraystar
agilent
SPSS
ImageJ
genespring gx

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