LncRNA PTCSC3/miR-574-5p Governs Cell Proliferation and Migration of Papillary Thyroid Carcinoma via Wnt/β-Catenin Signaling

Journal of Cellular Biochemistry
Xiaoming WangYang Shi

Abstract

The distance metastases of papillary thyroid carcinoma (PTC) were a major threaten for PTC patients, thus, to study the potential mechanism for the treatment of PTC was essential. Previous studies have shown that PTCSC3 (Thyroid Carcinoma Susceptibility Candidate 3), miR-574-5p and Wnt/β-catenin were involved in PTC, but the potential pathogenic mechanism among them was still unclear. Real-time PCR and Western blot were used to detect genes expression. Luciferase reporter assay was used to detect the combination of miR-574-5p and suppressor of cancer cell invasion (SCAI), as well as the ratio of TOP/FOP. RNA Pull-down assay verified the bound of PTCSC3 and miR-574-5p. MTT assay, Transwell assay, and wound scratch assay were used to detect cell viability and cell migration. The expression of PTCSC3 and SCAI were decreased, while miR-574-5p and β-catenin were increased in PTC tissues and cells. Overexpressed PTCSC3 suppressed cell proliferation and migration, promoted the expression of SCAI, but inhibited β-catenin. PTCSC3 absorbed miR-574-5p, and miR-574-5p targeted to SCAI; SCAI could regulate the activity of Wnt/β-catenin. PTCSC3/miR-574-5p regulated the activity of Wnt/β-catenin via SCAI and mediated cell proliferation and mi...Continue Reading

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Citations

Mar 22, 2018·Cancer Biology & Therapy·Xiao-Ming WangXiu-Bo Lu
Mar 19, 2019·Journal of Cellular Physiology·Zheng LiJianxiong Shen
Apr 27, 2019·Journal of Cellular Biochemistry·Ning WangXiaobin Sheng
Feb 21, 2019·Journal of Cellular Physiology·Haifeng YuDapang Rao
Sep 24, 2019·Journal of Cellular Biochemistry·Yiping ZhuXiaoping Qian
Oct 7, 2019·Clinical and Experimental Pharmacology & Physiology·Rui TongLing Wang
Nov 9, 2019·Cancer Biotherapy & Radiopharmaceuticals·Guodong ZhangYongzhi Zhuang
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Jul 1, 2020·Frontiers in Oncology·Xuejiao PengWeiqin Chang
Dec 19, 2019·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Zicheng WeiXiaoming Liu
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Dec 16, 2020·Technology in Cancer Research & Treatment·Zhonghao LinHuiqing Ruan

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