LncRNA PVT1 regulates ferroptosis through miR-214-mediated TFR1 and p53

Life Sciences
Jingjing LuHong Lu

Abstract

The study aims to investigate the roles of LncRNA and miRNA in ferroptosis in brain ischemia/reperfusion (I/R) in vivo and in vitro. qPCR assay was used to analyze lncRNA PVT1 and miR-214 expressions in acute ischemic stroke (AIS) patients. Then, we established brain I/R mice models and OGD/R PC12 cell models to analyze the mechanism of ferroptosis. I/R mice were treated by lncRNA PVT silencing or miR-214 overexpressing lentivirus via lateral ventricles. Infarct size was analyzed by TTC staining, accompanied by the detection of ferroptosis indicators through Perls'Prussian blue staining, iron kit, MDA kit, glutathione kit, GPx activities kit and Western blotting (WB). Dual luciferase reporter assay was used to assess whether miR-214 bound to PVT1, TP53 or TFR1. Co-IP analyzed the interplay of p53 with SLC7A11. We found that the levels of PVT1 were upregulated and miR-214 levels were downregulated in plasma of AIS patients. NIHSS score was positively correlated with PVT1 levels but was negatively with miR-214 levels. PVT1 silencing or miR-214 overexpression significantly reduced infarct size and suppressed ferroptosis in vivo. miR-214 overexpression markedly decreased PVT1 levels. Specifically, miR-214 could bind to 3'untranslat...Continue Reading

Citations

Feb 13, 2021·Oxidative Medicine and Cellular Longevity·Yue ChengXiaojing Liu
Feb 9, 2021·Frontiers in Cell and Developmental Biology·Xin ChenRui Kang
Nov 14, 2020·International Journal of Molecular Sciences·Juan LiuZhaohui Feng
Apr 1, 2021·Seminars in Cancer Biology·Yanqing Liu, Wei Gu
May 1, 2021·International Journal of Molecular Sciences·Chisato Kinoshita, Koji Aoyama
Jun 8, 2021·Frontiers in Cell and Developmental Biology·Jianglin ZhengXiaobing Jiang
Jun 14, 2021·International Journal of Biological Macromolecules·Haipeng WangLing Zhang
Jul 15, 2021·Oxidative Medicine and Cellular Longevity·Yue ZhouJinwen Ge
Jul 18, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Bingwei ZhangKe Tan
Aug 13, 2021·Cell Death Discovery·Yan LiuZuozhang Yang
Aug 27, 2021·Bioorganic & Medicinal Chemistry Letters·Xue LiGang Chen

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