LncRNA SNHG1 alleviates IL-1β-induced osteoarthritis by inhibiting miR-16-5p-mediated p38 MAPK and NF-κB signaling pathways

Bioscience Reports
Jinlai LeiDaigang Lu

Abstract

Long non-coding RNA (LncRNA) small nucleolar RNA host gene 1 (SNHG1) has been reported in the occurrence and development of several diseases, but its biological role and mechanism in osteoarthritis (OA) remain to be illuminated. In the present research, we aimed to investigate the effect of SNHG1 on IL-1β-induced OA and its molecular mechanism. Results revealed that SNHG1 decreased the expression of MMPs, ADAMTs, collagen, and aggrecan, and ameliorates IL-1β-induced metabolic dysfunction in normal human chondrocytes-keen. In addition, SNHG1 inhibited the expressions of pro-inflammatory cytokines in chondrocytes, including NO, PGE2, IL-6, TNF-α, i-NOS, and COX-2. Furthermore, luciferase reporter assay demonstrated that SNHG1 could directly interact with miR-16-5p and suppressed miR-16-5p expression and activity. What is more, miR-16-5p overexpression reversed SNHG1-inhibited aberrant catabolism and inflammation triggered by IL-1β stimulation. Finally, SNHG1 inhibits the expression of miR-16-5p-mediated factors involved in p38MAPK and NF-κB signaling pathways, including ERK1/2, p-p38 and p-p65. Taken together, the results of our studies illuminate that SNHG1 alleviates the inflammation of IL-1β-induced OA through the activation o...Continue Reading

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Citations

Jan 14, 2020·BioMed Research International·Xinjie WuMingsheng Tan
Apr 17, 2020·Journal of Molecular Histology·Liping ZhangYutao Wu
Feb 2, 2021·Frontiers in Bioengineering and Biotechnology·Olanrewaju B MorenikejiBolaji N Thomas
Jan 15, 2021·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Federica ReyStephana Carelli
Dec 20, 2020·International Journal of Molecular Sciences·Leticia Moreno-GarcíaRosario Osta
Feb 13, 2020·Cardiovascular Research·Bruna Lima CorreaJean-Sébastien Silvestre
Mar 24, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Taheri
Oct 1, 2021·Nature Reviews. Rheumatology·Shabana A AliMohit Kapoor
Dec 11, 2021·Frontiers in Pharmacology·Jinxia DengBo Wei

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Methods Mentioned

BETA
transfection
zymography
Assay
immunoprecipitation
RIP
PCR
ELISA
immunoprecipitation assay

Software Mentioned

microRNA software (
ImageJ
SPSS

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