LncRNA SNHG16 drives proliferation, migration, and invasion of hemangioma endothelial cell through modulation of miR-520d-3p/STAT3 axis

Cancer Medicine
Wenke ZhaoYang Liu

Abstract

It has been verified that long noncoding RNAs (lncRNAs) have great effects on various biological behaviors of human diseases. Although more and more lncRNAs have been studied in human cancers, countless lncRNAs still need to be excavated. This study aims to investigate the impacts of lncRNA SNHG16 on proliferation and metastasis of human hemangioma endothelial cell (HemECs). qRT-PCR analysis was carried out to explore the expression pattern of SNHG16, miR-520d-3p, and STAT3. The effect of SNHG16 on cell proliferation was detected by MTT and colony formation assay. Flow cytometry analysis was performed to test the apoptosis of HemECs cells. Migration and invasion of HemECs cells were determined and examined by transwell assays. Tube formation assay helped to observe the influence of SNHG16 expression on the vasoformation of HemECs cells. The correlations among SNHG16, miR-520d-3p, and STAT3 were certified by bioinformatics analysis, pull-down assay, and dual-luciferase reporter assay. Finally, rescue assays were conducted to demonstrate the effects of SNHG16-miR-520d-3p-STAT3 axis on biological behaviors of HemECs cell. SNHG16 was strongly expressed in proliferating phase hemangioma tissues and HemECs cells. Silenced SNHG16 nega...Continue Reading

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Citations

Jan 10, 2019·Journal of Cellular Physiology·Renjie ChiHongting Lu
Jan 12, 2019·Human Gene Therapy. Clinical Development·Liang HanXudong Wu
Apr 13, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Haochuan LiuQingsan Zhu
Sep 29, 2019·Cancer Biomarkers : Section a of Disease Markers·Chunhuan ZhouHai Huang
Mar 30, 2019·Cancer Chemotherapy and Pharmacology·Shian LiaoYanhui Ma
May 8, 2020·Computational and Mathematical Methods in Medicine·Yuansheng RaoJianhong Wang
Dec 29, 2020·Experimental and Molecular Pathology·Lin HuangZhigang You
Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yun XiangJiayun Zhang
Jun 10, 2021·Laboratory Investigation; a Journal of Technical Methods and Pathology·Qian-Qian ZhuHong-Kun Zhang
Oct 22, 2021·Frontiers in Cell and Developmental Biology·Soudeh Ghafouri-FardSeyedpouzhia Shojaei
Jan 14, 2022·Annals of Plastic Surgery·Yuming SunShaorong Lei

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Methods Mentioned

BETA
immunoprecipitation
RIP
transfect
flow cytometry
flow
transfection
fractionation
transfecting

Software Mentioned

SPSS
Image J

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