LncRNA SNHG16 functions as an oncogene by sponging miR-200a-3p in pancreatic cancer

European Review for Medical and Pharmacological Sciences
J-Q GuoD-C Wang

Abstract

Recently, the role of long noncoding RNAs (lncRNAs) is vital in tumor progression. Our study aims to identify the role of SNHG16 in the metastasis of pancreatic carcinoma. Real-Time quantitative Polymerase Chain Reaction (RT-qPCR) was used to measure SNHG16 expression in 56 pancreatic carcinoma patients' tissues. Function assays, including wound healing assay, and transwell assay, were conducted to detect the effect of SNHG16 on the metastasis of pancreatic carcinoma. Besides, the luciferase assay was performed to explore the underlying mechanism. The expression level of SNHG16 was upregulated in pancreatic carcinoma samples compared with adjacent tissues. Moreover, cell migration and cell invasion were repressed via the knockdown of SNHG16, while cell migration and cell invasion were promoted via the overexpression of SNHG16. Moreover, the expression of miR-200a-3p was upregulated via knockdown of SNHG16 while the expression of miR-200a-3p was downregulated via the upregulation of SNHG16 in vitro. Furthermore, it was discovered that SNHG16 acted as a competing endogenous RNA via sponging miR-200a-3p in pancreatic carcinoma. Our study suggests that SNHG16 acts as an oncogene in pancreatic carcinoma and promotes cell metastasis ...Continue Reading

Citations

Jun 18, 2020·Biology·Milad AshrafizadehReza Mohammadinejad
Aug 28, 2020·OncoTargets and Therapy·Yuan QinHao Zhang

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