LncRNA SNHG16 regulates trophoblast functions by the miR-218-5p/LASP1 axis.

Journal of Molecular Histology
Zhou YuHaidong Wang

Abstract

Altered placental development and function lead to placental diseases such as preeclampsia (PE) which is mainly characterized by insufficient trophoblast invasion and abnormally invasive placenta disorders. Long noncoding RNAs (lncRNAs) are widely reported to function as crucial players in the pathogenesis of PE. The present investigation clarified the role of lncRNA small nucleolar RNA host gene 16 (SNHG16) in PE. RT-qPCR was used to measure gene expression. The proliferation of trophoblast cells was examined using CCK-8 and EdU assays. Trophoblast migration and invasion were assessed using wound healing and transwell assays. The apoptosis was estimated by flow cytometry. Luciferase reporter and RNA pull-down assays were performed to explore the molecular mechanisms in trophoblast cells. We found that SNHG16 was downregulated in placenta from patients with PE. Moreover, SNHG16 depletion significantly inhibited trophoblast cell proliferation, migration, and invasion and stimulated apoptosis, while SNHG16 overexpression exerted an opposite effect. Subsequently, we confirmed that SNHG16 acted as a competing RNA (ceRNA) of miR-218-5p that was verified to directly target LASP1. Both miR-218-5p depletion and LASP1 upregulation antag...Continue Reading

References

Jun 11, 2005·Science·Christopher W Redman, Ian L Sargent
Jan 17, 2014·Nature·Yvonne TayPier Paolo Pandolfi
Jan 31, 2015·Current Opinion in Nephrology and Hypertension·Monica SircarS Ananth Karumanchi
Apr 15, 2015·Trends in Genetics : TIG·Georges St LaurentPhilipp Kapranov
Sep 8, 2015·Lancet·Ben W J MolG Justus Hofmeyr
Nov 26, 2015·Pregnancy Hypertension·George F GuirguisLeena P Shah
Jan 29, 2016·Current Opinion in Genetics & Development·Eric A Alcid, Toshio Tsukiyama
Oct 30, 2016·Microvascular Research·Nisha HariharanStephen Wagner
Nov 11, 2016·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Takuji TomimatsuTadashi Kimura
Nov 23, 2016·Advances in Experimental Medicine and Biology·Asif AhmedSophie Broadway-Stringer
Apr 17, 2017·The International Journal of Biochemistry & Cell Biology·Min FangTao Zhang
Nov 1, 2017·Genome Biology·Francesco P MarcheseMaite Huarte
Mar 30, 2019·Cancer Chemotherapy and Pharmacology·Shian LiaoYanhui Ma
May 31, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Chuanhong ZhongLigang Chen
Nov 7, 2019·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Xuehui ZhaoXiaowen Li
Jul 25, 2020·European Review for Medical and Pharmacological Sciences·S-B LiuB Hu
Oct 6, 2020·European Review for Medical and Pharmacological Sciences·L ChenM Zhang
Nov 25, 2020·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·Utku AkgörFiliz Çayan
Dec 29, 2020·Experimental and Molecular Pathology·Lin HuangZhigang You

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.