LncRNA SNHG7 promotes the proliferation of esophageal cancer cells and inhibits its apoptosis

European Review for Medical and Pharmacological Sciences
L-J XuX-F Chen

Abstract

Our research studied the expression of long noncoding RNA (lncRNA) SNHG7 in esophageal cancer cells and tissues. The effect of lncRNA SNHG7 on proliferation and apoptosis of esophageal cancer cells has been discussed. Si-SNHG7 was transfected into esophageal cancer cells, and qRT-PCR was performed to detect the expression of lncRNA SNHG7 in esophageal cancer cells and tissues. The effect of SNHG7 on the proliferation of esophageal cancer cells was measured by CCK8 assay and plate cloning assay, respectively. Flow cytometry was used to detect the effect of SNHG7 on the cell cycle and apoptosis rate of esophageal cancer cells. Changes in expression of downstream protein p15 and p16 after si-SNHG7 intervention were analyzed by qRT-PCR and Western blot. QRT-PCR showed that the expression of SNHG7 in esophageal cancer tissues and cells was significantly up-regulated. After the si-SNHG7 intervention, the proliferation of esophageal cancer cells was inhibited, the apoptosis rate increased, and the cell cycle was blocked in G1-G0 phase. QRT-PCR and Western blot showed that, after the si-SNHG7 intervention, the expression of p15 and p16 increased significantly. The expression of SNHG7 in the tissues and cells of esophageal cancer is sig...Continue Reading

Citations

Apr 23, 2020·Frontiers in Oncology·Alina-Andreea ZimtaIoana Berindan-Neagoe
Jan 8, 2021·OncoTargets and Therapy·Weifeng ZhaoChaojie Wang
Jul 28, 2021·Digestive Diseases and Sciences·Jindun Pan, Yunhong Zang

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