lncRNA STEAP3-AS1 Modulates Cell Cycle Progression via Affecting CDKN1C Expression through STEAP3 in Colon Cancer

Molecular Therapy. Nucleic Acids
Heya NaYunfei Zuo

Abstract

Previous studies have reported that long noncoding RNAs (lncRNAs) have acted as new players during tumorigenesis. Metallothionein also plays an important role in tumor progression. It is mainly considered to be involved in the process of cell proliferation, oxidative stress, and multidrug resistance. However, the potential involvement of metallothionein-related lncRNAs in colon cancer remains poorly understood. In our study, we found that MT1M affected the expression of lncRNA STEAP3-AS1. STEAP3-AS1 is located in physical contiguity with STEAP3 and notably increased in colon cancer tissues and cell lines. STEAP3-AS1 expression was negatively associated with the expression of STEAP3. High levels of STEPA3-AS1 were associated with poor overall survival in colon cancer patients. In in vitro assays, STEAP3-AS1 knockdown could inhibit colon cancer cell proliferation and migration and arrest colon cancer cells at the G0-G1 phase. In tumorigenicity assays, STEAP3-AS1 knockdown could strongly inhibit tumor growth. Mechanistic investigations demonstrated that STEAP3-AS1 downregulation could increase the expression of cyclin-dependent kinase inhibitor 1C (CDKN1C) by STEAP3 upregulation. Overall, we identify the underlying role of MT1M-re...Continue Reading

Citations

Dec 29, 2020·Cancer Letters·Arun NandwaniMalabika Datta
Dec 18, 2020·Frontiers in Oncology·Soudeh Ghafouri-FardMohammad Taheri
Oct 31, 2021·Journal of Clinical Laboratory Analysis·Xiao JingShouhua Pan

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Methods Mentioned

BETA
PCR
to
transfection
flow cytometry
immunoprecipitation
RIP

Software Mentioned

Arraystar
catRAPID
ImageQuant LAS 500
LncPro

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