LncRNA TUG1 promoted KIAA1199 expression via miR-600 to accelerate cell metastasis and epithelial-mesenchymal transition in colorectal cancer

Journal of Experimental & Clinical Cancer Research : CR
Junfeng SunJiaxiang Wang

Abstract

LncRNA TUG1 has been reported to be highly expressed in CRC samples and cells and promoted metastasis by affecting EMT, indicating a poor prognosis for colorectal cancer (CRC). In this study, we determined the underlying mechanism for tumor oncogenesis of lncRNA TUG1 in CRC metastasis. The expressions of miR-600 and KIAA1199 in 76 CRC patients and CRC cells and CRC metastatic tissues were determined using qRT-PCR. Epithelial-mesenchymal transition (EMT)-related proteins were determined using western blot. CRC cell metastasis was assessed by colony formation, wound healing and transwell assay. Luciferase reporter gene assay was used to confirm miR-600 binding to KIAA1199 3'UTR. Our data showed that lncRNA TUG1 was upregulated in CRC cells, miR-600 was downregulated in CRC tissues, cell lines and CRC metastatic tissues, and low miR-600 expression predicted a poor clinical prognosis. Overexpression of miR-600 suppressed CRC cell migration/invasion and EMT-related proteins in vitro, inhibited tumor volume and weight, and decreased the number of CRC liver metastasis in vivo. KIAA1199 was upregulated in CRC tissues, and was negatively regulated by miR-600. KIAA1199 overexpression promoted CRC cell migration and invasion, which revers...Continue Reading

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Citations

Mar 27, 2019·Journal of Cellular Physiology·Soudeh Ghaforui-FardMohammad Taheri
Jun 21, 2019·Journal of Cellular Biochemistry·Jianbo GuoJingang Liu
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Datasets Mentioned

BETA
KIAA1199
GSE21510

Methods Mentioned

BETA
PCR
Immunoprecipitation
Assay
Protein Assay
electrophoresis
RIP
xenografts

Software Mentioned

Targetscan
DIANA
Image J
SPSS

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