LncRNA uc.48+ is involved in the diabetic immune and inflammatory responses mediated by P2X7 receptor in RAW264.7 macrophages

International Journal of Molecular Medicine
Hong WuYijun Nie

Abstract

High glucose combined with high FFAs can contribute to the unfavorable development of type 2 diabetes mellitus (T2DM) and monocytes/macrophages are important in the occurrence and development of T2DM, which is regarded as a type of low‑grade inflammation. Although our previous study demonstrated that increased expression of P2X7 receptor (P2X7R) in peripheral blood monocytes may alter the innate immune system and that long non‑coding (lnc)RNA uc.48+ was involved in diabetic neuropathic pain, the involvement of uc.48+ mediated by the P2X7R in monocyte/macrophages during T2DM has not been reported. In the present study, the effectsof uc.48+ small interference RNA (siRNA) on factors, including the mRNA and protein expression of P2X7R, apoptosis and proliferation, levels of reactive oxygen species (ROS), cytokine levels, and expression of phosphorylated (p‑) extracellular signal‑regulated kinase (ERK)1/2, were examined in RAW264.7 macrophages following exposure to high glucose and high plasma free fatty acids (FFAs). After RAW264.7 cells were transfected with uc.48+ siRNA under high glucose conditions and FFAs treatment, the mRNA expression levels of uc.48+ and P2X7 receptor were detected by reverse transcription‑polymerase chain r...Continue Reading

References

May 8, 2004·Science·Gill BejeranoDavid Haussler
May 25, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Anna SoliniFrancesco Di Virgilio
Mar 24, 2009·Annual Review of Immunology·Charles A Dinarello
Jan 16, 2010·The Journal of Biological Chemistry·Anders BoysenMartin Overgaard
Dec 17, 2010·Journal of Internal Medicine·C A Dinarello
Jun 17, 2011·American Journal of Physiology. Renal Physiology·Murugavel PonnusamyShougang Zhuang
Nov 30, 2011·Diabetologia·J M Fernández-Real, J C Pickup
Jul 31, 2012·Microbes and Infection·Ceren CiraciSuzanne L Cassel
Apr 3, 2013·Purinergic Signalling·Geoffrey Burnstock, Ivana Novak
Aug 31, 2013·Metabolism: Clinical and Experimental·Viswanathan SaraswathiDale S Edgerton
Apr 18, 2014·Anesthesiology·Brianna Marie LutzYuan-Xiang Tao
May 7, 2014·Diabetes Research and Clinical Practice·Nathalie EsserNicolas Paquot
Jun 25, 2014·Annals of Agricultural and Environmental Medicine : AAEM·Łucja Czyżewska-MajchrzakHenryk Witmanowski
Jan 17, 2015·Pflügers Archiv : European journal of physiology·Michaela StolzFritz Markwardt
Mar 31, 2015·Circulation Research·Nicolas Jaé, Stefanie Dimmeler
May 24, 2015·Journal of Cardiovascular Medicine·Wei GaoLian-Sheng Wang
Aug 13, 2015·Purinergic Signalling·Rafael Fernandes ZaninAna Maria Oliveira Battastini
Apr 28, 2016·Autonomic Neuroscience : Basic & Clinical·Bing WuShangdong Liang
Aug 26, 2016·Immunology and Cell Biology·Szu-Wei HuangSheena M Cruickshank

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.