LncRNA XIST promotes the progression of laryngeal squamous cell carcinoma via sponging miR-125b-5p to modulate TRIB2.

Bioscience Reports
Chunxiu LiuPing Guo

Abstract

X inactivate-specific transcript (XIST) is an attractive long noncoding RNA (lncRNA) functioning as an indicator of various human tumors, including laryngeal squamous cell carcinoma (LSCC). The present study was conducted to explore a novel regulatory network of lncRNA XIST in LSCC cells. Quantitative real-time polymerase chain reaction (QRT-PCR) was used to detect the expression levels of XIST, miR-125b-5p and TRIB2 in LSCC cells and tissues. Cell proliferation, apoptosis, migration and invasion were detected by Cell Counting Kit-8 (CCK-8), flow cytometry and Transwell assays, separately. The relationship among XIST, miR-125b-5p and tribbles homolog 2 (TRIB2) was predicted by starBase v2.0 or TargetScan and confirmed by Dual-luciferase reporter assay. The TRIB2 protein expression was quantified by Western blot assay. Murine xenograft model was utilized to validate the role of XIST in vivo. XIST was notably up-regulated in LSCC tissues and cells, and the high level of XIST was associated with the low survival rate of LSCC patients. XIST knockdown markedly repressed cell proliferation, migration and invasion and promoted the apoptosis of LSCC cells and the effects were antagonized by loss of miR-125b-5p. MiR-125b-5p was a target...Continue Reading

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Citations

Apr 3, 2021·Cell Communication and Signaling : CCS·Yu FangSeyed Esmaeil Khoshnam
Jun 29, 2021·Frontiers in Cell and Developmental Biology·Soudeh Ghafouri-FardSeyed Ali Mousavinejad

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Methods Mentioned

BETA
transfection
PCR
Assay
electrophoresis
flow cytometry
xenograft

Software Mentioned

TargetScan
SPSS
XIST
Quantity One
GraphPad Prism

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