lncRNA XIST regulates proliferation and migration of hepatocellular carcinoma cells by acting as miR-497-5p molecular sponge and targeting PDCD4

Cancer Cell International
Yixi ZhangXiaoshun He

Abstract

MicroRNAs (miRNAs) play a pivotal role in hepatocellular carcinoma (HCC) progression and have been confirmed to participate in the carcinogenesis and development of HCC. However, the relationship between miR-497-5p and HCC remains unclear. Kaplan-Meier curve analysis and the log-rank test were used to investigate the efficacy of miR-497-5p on overall survival (OS) and disease-free survival (DFS) in patients with HCC. According to in vitro experiments, programmed cell death 4 (PDCD4) was a target of miR-497-5p by the dual-luciferase activity assay. The efficacy of PDCD4 on cell proliferation and metastasis in HCC was examined by transwell assays, CCK-8 assays and reverse transcription quantitative PCR (RT-qPCR). Additionally, we conducted a luciferase activity reporter assay to confirm the interaction between lncRNA XIST and miR-49-5p. Then, to evaluate the relationship between lncRNA XIST and miR-497-5p, several mechanistic experiments, including qRT-PCR, Western blotting, transwell assays and tumor xenograft assays, were performed. miR-497-5p was upregulated in HCC tissues, and high expression of miR-497-5p resulted in increases in tumor size and tumor number and a higher tumor-node-metastasis (TNM) stage and Edmondson grade i...Continue Reading

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Citations

Oct 12, 2019·Clinica Chimica Acta; International Journal of Clinical Chemistry·Dandan LiJinming Li
Oct 17, 2020·Cancer Cell International·Gang XuZhong-Tao Zhang
Apr 21, 2020·Current Pharmaceutical Biotechnology·Atieh Hashemi, Gilar Gorji-Bahri
Jun 8, 2021·BioMed Research International·Yujie FengChuandong Sun
Jun 29, 2021·Frontiers in Cell and Developmental Biology·Soudeh Ghafouri-FardSeyed Ali Mousavinejad
Jun 29, 2021·Frontiers in Cell and Developmental Biology·Wenlun WangLingyun Zhu

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Methods Mentioned

BETA
PCR
flow cytometry
FACS
transfection
xenograft
xenografts

Software Mentioned

miRanda
SPSS

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