Loading of iron into recombinant rat liver ferritin heteropolymers by ceruloplasmin

Archives of Biochemistry and Biophysics
S H JuanS D Aust

Abstract

We have reported previously that the heavy chain of ferritin is required for iron incorporation by ceruloplasmin (J.-H. Guo, M. Abedi, and S. D. Aust (1996) Arch. Biochem. Biophys. 335(1)). The purpose of this study was to determine how many heavy chains were required for ceruloplasmin to interact with ferritin such that iron loading occurred. The cDNA sequences encoding the heavy and light chains of rat liver ferritin were cloned into the baculovirus transfer vector pA-cUW51 under the control of polyhedrin and p10 promoters, respectively, which was then incorporated by homologous recombination into the infections Autographa californica nuclear polyhedrosis virus genome. Both ferritin chains were expressed and assembled into two heteropolymers following the infection of insect cells by recombinant virus, which were separated by DEAE-Sepharose chromatography. The percentage of heavy (H) and light (L) chains making up the two heteropolymers, determined by gel scanning following the resolution of chains on SDS-PAGE, were equivalent to 1 H and 23 L chains and 2 H and 22 L chains. The maximal extent of iron loading was observed using 1 mol of rat ceruloplasmin per mole of H chain in the two heteropolymers. The extent of iron incorpo...Continue Reading

References

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Feb 14, 1992·Archives of Biochemistry and Biophysics·T P RyanS D Aust
Mar 1, 1992·Archives of Biochemistry and Biophysics·D de SilvaS D Aust
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Citations

Jan 20, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Kevin D WelchSteven D Aust
Jul 27, 2001·Brain Research Bulletin·K J ThompsonJ R Connor
Aug 25, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Seong Ryul KimByung Rae Jin
Jun 2, 2000·Protein Expression and Purification·P SantambrogioP Arosio
Jan 9, 2001·Archives of Biochemistry and Biophysics·J E GraceS D Aust
Oct 6, 2000·Archives of Biochemistry and Biophysics·M E Van Eden, S D Aust
Jan 12, 1999·Archives of Biochemistry and Biophysics·S H Juan, S D Aust
Dec 31, 1997·Archives of Biochemistry and Biophysics·C A Reilly, S D Aust
Jun 20, 1998·Archives of Biochemistry and Biophysics·C A ReillyS D Aust
May 9, 1998·Archives of Biochemistry and Biophysics·J H GuoS D Aust
Sep 15, 1998·Archives of Biochemistry and Biophysics·S H Juan, S D Aust
Jul 2, 1998·Archives of Biochemistry and Biophysics·S H Juan, S D Aust
Oct 12, 2001·Free Radical Biology & Medicine·K D WelchS D Aust

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