Local action of long-range repressors in the Drosophila embryo

The EMBO Journal
Yutaka NibuM Levine

Abstract

Previous studies have identified two corepressors in the early Drosophila embryo: Groucho and dCTBP: Both proteins are recruited to the DNA template by interacting with short peptide motifs conserved in a variety of sequence-specific transcriptional repressors. Once bound to DNA, Groucho appears to mediate long-range repression, while dCtBP directs short-range repression. The short-range Krüppel repressor was converted into a long-range repressor by replacing the dCtBP interaction motif (PxDLSxH) with a Groucho motif (WRPW). The resulting chimeric repressor causes a different mutant phenotype from that of the native Krüppel protein when misexpressed in transgenic embryos. The different patterning activities can be explained on the basis of long-range silencing within the hairy 5' regulatory region. The analysis of a variety of synthetic transgenes provides evidence that Groucho-dependent long-range repressors do not always cause the dominant silencing of linked enhancers within a complex cis-regulatory region. We suggest a "hot chromatin" model, whereby repressors require activators to bind DNA.

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Citations

Oct 12, 2010·Cellular and Molecular Life Sciences : CMLS·Johannes Jaeger
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sandhya Payankaulam, David N Arnosti
Dec 11, 2013·Molecular and Cellular Biology·Benjamin E HousdenSarah J Bray
Feb 21, 2008·Molecular and Cellular Biology·Carlos A Martinez, David N Arnosti
May 16, 2003·Molecular and Cellular Biology·Yutaka NibuMichael Levine
Apr 16, 2005·Molecular and Cellular Biology·Meghana M Kulkarni, David N Arnosti
Mar 23, 2010·PLoS Biology·János Z KelemenAttila Becskei
Apr 20, 2007·Proceedings of the National Academy of Sciences of the United States of America·Gregory J Kubas
Nov 24, 2007·EMBO Reports·Barbara H JenningsDavid Ish-Horowicz
Jan 21, 2015·Transcription·Aamna K KaulBarbara H Jennings
Aug 26, 2015·ELife·Kurtulus KokDavid N Arnosti
Aug 7, 2008·Current Biology : CB·Sandhya Payankaulam, David N Arnosti
Mar 26, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jacqueline HoyleHazel Sive
Nov 13, 2004·Development·Stephanie E Winter, Gerard Campbell

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