PMID: 7517326Mar 1, 1994Paper

Local anaesthetic blockade of neuronal nicotinic ACh receptor-channels in rat parasympathetic ganglion cells

British Journal of Pharmacology
J Cuevas, D J Adams

Abstract

1 The effects of the local anaesthetics QX-222 and procaine on nicotinic acetylcholine (ACh)-evoked currents in cultured parasympathetic cardiac neurones of the rat were investigated by use of the whole-cell, perforated-patch, and outside-out recording configurations of the patch clamp method. 2 QX-222 and procaine, applied to the extracellular surface, reversibly inhibited the peak amplitude of the whole-cell nicotinic ACh-evoked current in a concentration-dependent manner, with half-maximal inhibitory concentrations (IC50) of 28 microM and 2.8 microM, respectively, at -80 mV. In these neurones, the sustained inward current mediated by M1 muscarinic receptor activation was unaltered by QX-222, and neither local anaesthetic affected the adenosine 5'-triphosphate (ATP)-evoked current. 3 QX-222 and procaine block of nicotinic ACh-evoked inward current was voltage-dependent and enhanced by hyperpolarization. An e-fold change in their dissociation equilibrium constants (Kd) resulted from a 62 mV and a 122 mV change in membrane potential, respectively. 4 Both local anaesthetics produce a concentration-dependent increase in the half-time of decay of the nicotinic ACh-evoked inward current. 5 Measurements of unitary currents in outsid...Continue Reading

References

Sep 1, 1976·The Journal of Physiology·P R Adams
Jun 1, 1977·The Journal of Physiology·P R Adams
Apr 1, 1978·The Journal of Physiology·E Neher, J H Steinbach
Aug 1, 1992·Journal of the Autonomic Nervous System·A A Selyanko, V I Skok
Aug 1, 1992·British Journal of Pharmacology·P CharlesworthC D Richards
Jan 1, 1992·International Review of Neurobiology·R J Lukas, M Bencherif
Jan 1, 1991·Trends in Pharmacological Sciences·E S DenerisR Duvoisin
Aug 1, 1990·Anesthesia and Analgesia·M Tabatabai, A M Booth
Jun 1, 1990·British Journal of Pharmacology·T G Allen, G Burnstock
Aug 1, 1988·The Journal of General Physiology·R Horn, A Marty
Jun 1, 1973·The Journal of General Physiology·A M Woodhull
Feb 1, 1984·The Journal of Physiology·D E Clapham, E Neher
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Feb 1, 1983·The Journal of Physiology·P W GageR E Wachtel
Jan 1, 1993·Annual Review of Neuroscience·P B Sargent
Mar 1, 1952·Journal of Neurophysiology·M G LARRABEE, J M POSTERNAK

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Citations

Apr 15, 1996·Journal of Neuroscience Research·H R Arias
Dec 11, 2002·Biochemical Pharmacology·Tae-Ju ParkKyong-Tai Kim
Dec 14, 1999·Neuroscience and Biobehavioral Reviews·H R Arias
Dec 24, 1997·Brain Research. Brain Research Reviews·H R Arias
Mar 5, 1999·Brain Research. Developmental Brain Research·J E HengE B Dreyer
May 31, 2002·Autonomic Neuroscience : Basic & Clinical·Vladimir I Skok
Nov 22, 2007·The European Journal of Neuroscience·Jean-Marc DevaudBernd Grünewald
Feb 22, 2011·Autonomic & Autacoid Pharmacology·Y OkunoT Kitazawa
Oct 1, 2015·Pharmacological Reviews·Daniel BertrandDiana Donnelly-Roberts
Mar 15, 2005·The Journal of Pharmacology and Experimental Therapeutics·Hongling Zhang, Javier Cuevas
Nov 1, 1996·Canadian Journal of Physiology and Pharmacology·W Y Lu, D Bieger
Jul 8, 2016·The Journal of Biological Chemistry·Tommy S TillmanPei Tang

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