Local and propagated dendritic action potentials evoked by glutamate iontophoresis on rat neocortical pyramidal neurons

Journal of Neurophysiology
P Schwindt, W Crill

Abstract

Iontophoresis of glutamate at sites on the apical dendrite 278-555 microm from the somata of rat neocortical pyramidal neurons evoked low-threshold, small, slow spikes and/or large, fast spikes in 71% of recorded cells. The amplitude of the small, slow spikes recorded at the soma averaged 9.1 mV, and their apparent threshold was <10 mV positive to resting potential. Both their amplitude and their apparent threshold decreased as the iontophoretic site was moved farther from the soma. These spikes were not abolished by somatic hyperpolarization. When the somata of cells displaying these small spikes were voltage clamped at membrane potentials that prevented somatic or axonic firing, corresponding current spikes could be evoked all-or-none by dendritic depolarization, indicating that the small, slow spikes arose in the dendrite. Similar responses were not observed during somatic depolarization evoked by current pulses or glutamate iontophoresis. These small, slow spikes were abolished by blocking voltage-gated Ca2+ channels but not by blocking Na+ channels or N-methyl-D-aspartate receptors. We conclude that these Ca2+ spikes occurred in a spatially restricted region of the dendrite and were not actively propagated to the soma. In ...Continue Reading

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Citations

May 20, 1998·Journal of Neurophysiology·P C Schwindt, W E Crill
Oct 20, 1999·Nature Neuroscience·F HelmchenD W Tank
Feb 28, 2003·The Journal of Pharmacology and Experimental Therapeutics·Wei-Xing Shi, Xue-Xiang Zhang
Feb 14, 2008·Nature Reviews. Neuroscience·Nelson Spruston
Apr 2, 2010·Journal of Neurophysiology·Bai-Chuang ShyuBrent A Vogt
Jul 27, 1999·Neural Computation·M A Kisley, G L Gerstein
May 11, 2005·Journal of Neurobiology·Andreas Frick, Daniel Johnston
Apr 9, 2008·Physiological Reviews·P Jesper SjöströmMichael Häusser
Sep 3, 2003·Nature Reviews. Neuroscience·Alain DestexheDenis Paré
Apr 2, 2011·PLoS Computational Biology·Tamar PashutAlon Korngreen
Jul 12, 2013·Annual Review of Neuroscience·Guy MajorJackie Schiller
Dec 22, 1999·Journal of Neurophysiology·D B Jaffe, N T Carnevale
Aug 25, 2001·Annual Review of Neuroscience·A Reyes
Dec 17, 2004·The Journal of Experimental Biology·Leonel GómezKirsty Grant
Dec 25, 2012·Journal of Neuroscience Methods·Gary J RoseChristopher J Leary

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